一种在黑腹果蝇中构建环状X染色体的简单快速方法。
A simple and rapid method for constructing ring-X chromosomes in Drosophila melanogaster.
作者信息
Golic Mary M, Golic Kent G
机构信息
Department of Biology, University of Utah, Salt Lake City, UT 84112, USA.
出版信息
Chromosoma. 2011 Apr;120(2):159-64. doi: 10.1007/s00412-010-0297-2. Epub 2010 Nov 18.
Abstract
Ring chromosomes are of basic interest to the geneticist and cell biologist who study their behavior in meiotic and mitotic divisions. In addition, the mitotic instability associated with some ring-X chromosomes has proven useful in Drosophila as a means to produce gynandromorphs for developmental studies. We describe a method to construct ring-X chromosomes in Drosophila via I-CreI-mediated exchange in rDNA, and then rapidly diagnose the recovery of ring chromosomes via FLP-mediated sister chromatid exchange within the ring. The method we describe provides a ready means to tailor the genetic content of ring-X chromosomes, making it suited to produce ring-X chromosomes for a variety of experimental purposes.
摘要
环形染色体对于研究其在减数分裂和有丝分裂中行为的遗传学家和细胞生物学家来说具有根本的研究意义。此外，与某些X环形染色体相关的有丝分裂不稳定性已被证明在果蝇中是有用的，可作为一种手段来产生用于发育研究的雌雄嵌合体。我们描述了一种通过rDNA中I-CreI介导的交换在果蝇中构建X环形染色体的方法，然后通过环内FLP介导的姐妹染色单体交换快速诊断环形染色体的回收情况。我们所描述的方法提供了一种现成的手段来定制X环形染色体的遗传内容，使其适合于为各种实验目的产生X环形染色体。
相似文献
1
A simple and rapid method for constructing ring-X chromosomes in Drosophila melanogaster.
Chromosoma. 2011 Apr;120(2):159-64. doi: 10.1007/s00412-010-0297-2. Epub 2010 Nov 18.
2
Ring chromosomes and rDNA magnification in Drosophila.
Genetics. 1984 Dec;108(4):969-83. doi: 10.1093/genetics/108.4.969.
3
Magnification in Drosophila: evidence for an inducible rDNA-specific recombination system.
Mol Gen Genet. 1994 Feb;242(3):321-6. doi: 10.1007/BF00280422.
4
Highly efficient sex chromosome interchanges produced by I-CreI expression in Drosophila.
Genetics. 2005 Nov;171(3):1103-14. doi: 10.1534/genetics.104.040071. Epub 2005 Jul 14.
5
Preferential Breakpoints in the Recovery of Broken Dicentric Chromosomes in Drosophila melanogaster.
Genetics. 2015 Oct;201(2):563-72. doi: 10.1534/genetics.115.181156. Epub 2015 Aug 20.
6
Magnification: gene amplification by an inducible system of sister chromatid exchange.
Trends Genet. 1988 Dec;4(12):348-51. doi: 10.1016/0168-9525(88)90055-8.
7
Evidence of P-element-induced sister-chromatid exchange in a ring-X chromosome in Drosophila, with implication for a high rate of formation of hybrid elements.
Genetics. 2006 Feb;172(2):975-9. doi: 10.1534/genetics.105.050609. Epub 2005 Sep 19.
8
Patterns of variation in the intergenic spacers of ribosomal DNA in Drosophila melanogaster support a model for genetic exchanges during X-Y pairing.
Genetics. 2000 Jul;155(3):1221-9. doi: 10.1093/genetics/155.3.1221.
9
The transmission of fragmented chromosomes in Drosophila melanogaster.
Genetics. 1998 Feb;148(2):775-92. doi: 10.1093/genetics/148.2.775.
10
Mitotic misbehavior of a Drosophila melanogaster satellite in ring chromosomes: insights into intragenomic conflict among heterochromatic sequences.
Fly (Austin). 2014;8(2):101-7. doi: 10.4161/fly.29488.
引用本文的文献
1
Acentric chromosome congression and alignment on the metaphase plate via kinetochore-independent forces.
Genetics. 2025 Feb 5;229(2). doi: 10.1093/genetics/iyae188.
2
Drosophila ring chromosomes interact with sisters and homologs to produce anaphase bridges in mitosis.
Genetics. 2024 Oct 25;228(4). doi: 10.1093/genetics/iyae169.
3
Drosophila ring chromosomes interact with sisters and homologs to produce anaphase bridges in mitosis.
bioRxiv. 2024 Aug 9:2024.08.08.607186. doi: 10.1101/2024.08.08.607186.
4
Dicentric chromosome breakage in Drosophila melanogaster is influenced by pericentric heterochromatin and occurs in nonconserved hotspots.
Genetics. 2023 May 26;224(2). doi: 10.1093/genetics/iyad052.
5
Kinetochore-independent mechanisms of sister chromosome separation.
PLoS Genet. 2021 Jan 29;17(1):e1009304. doi: 10.1371/journal.pgen.1009304. eCollection 2021 Jan.
6
Micronuclei Formation Is Prevented by Aurora B-Mediated Exclusion of HP1a from Late-Segregating Chromatin in .
Genetics. 2018 Sep;210(1):171-187. doi: 10.1534/genetics.118.301031. Epub 2018 Jul 9.
7
Preferential Breakpoints in the Recovery of Broken Dicentric Chromosomes in Drosophila melanogaster.
Genetics. 2015 Oct;201(2):563-72. doi: 10.1534/genetics.115.181156. Epub 2015 Aug 20.
8
Aurora B-mediated localized delays in nuclear envelope formation facilitate inclusion of late-segregating chromosome fragments.
Mol Biol Cell. 2015 Jun 15;26(12):2227-41. doi: 10.1091/mbc.E15-01-0026. Epub 2015 Apr 15.
9
Behavior of aberrant chromosome configurations in Drosophila melanogaster female meiosis I.
G3 (Bethesda). 2014 Dec 9;5(2):175-82. doi: 10.1534/g3.114.014316.
10
Mitotic misbehavior of a Drosophila melanogaster satellite in ring chromosomes: insights into intragenomic conflict among heterochromatic sequences.
Fly (Austin). 2014;8(2):101-7. doi: 10.4161/fly.29488.
本文引用的文献
1
Telomere loss provokes multiple pathways to apoptosis and produces genomic instability in Drosophila melanogaster.
Genetics. 2008 Dec;180(4):1821-32. doi: 10.1534/genetics.108.093625. Epub 2008 Oct 9.
2
The DrosDel deletion collection: a Drosophila genomewide chromosomal deficiency resource.
Genetics. 2007 Sep;177(1):615-29. doi: 10.1534/genetics.107.076216. Epub 2007 Aug 24.
3
A Cytological Study of W Chromosome Instability in Cleavage Mitoses of Drosophila Melanogaster.
Genetics. 1959 Sep;44(5):923-31. doi: 10.1093/genetics/44.5.923.
4
The Analysis of Rod Derivatives of an Unstable Ring Chromosome of Drosophila Melanogaster.
Genetics. 1957 Jan;42(1):55-65. doi: 10.1093/genetics/42.1.55.
5
The Behavior of an Unstable Ring Chromosome of Drosophila Melanogaster.
Genetics. 1955 Nov;40(6):951-61. doi: 10.1093/genetics/40.6.951.
6
Highly efficient sex chromosome interchanges produced by I-CreI expression in Drosophila.
Genetics. 2005 Nov;171(3):1103-14. doi: 10.1534/genetics.104.040071. Epub 2005 Jul 14.
7
The DrosDel collection: a set of P-element insertions for generating custom chromosomal aberrations in Drosophila melanogaster.
Genetics. 2004 Jun;167(2):797-813. doi: 10.1534/genetics.104.026658.
8
Systematic generation of high-resolution deletion coverage of the Drosophila melanogaster genome.
Nat Genet. 2004 Mar;36(3):288-92. doi: 10.1038/ng1312. Epub 2004 Feb 22.
9
MEIOTIC BEHAVIOR OF TANDEM ME TACENTRIC COMPOUND X CHROMOSOMES IN DROSOPHILA MELANOGASTER.
Genetics. 1965 Feb;51(2):223-45. doi: 10.1093/genetics/51.2.223.
10
Telomere loss in somatic cells of Drosophila causes cell cycle arrest and apoptosis.
Genetics. 1999 Mar;151(3):1041-51. doi: 10.1093/genetics/151.3.1041.
Zotero 插件