一种在黑腹果蝇中构建环状X染色体的简单快速方法。
A simple and rapid method for constructing ring-X chromosomes in Drosophila melanogaster.
作者信息
Golic Mary M, Golic Kent G
机构信息
Department of Biology, University of Utah, Salt Lake City, UT 84112, USA.
出版信息
Chromosoma. 2011 Apr;120(2):159-64. doi: 10.1007/s00412-010-0297-2. Epub 2010 Nov 18.
Abstract
Ring chromosomes are of basic interest to the geneticist and cell biologist who study their behavior in meiotic and mitotic divisions. In addition, the mitotic instability associated with some ring-X chromosomes has proven useful in Drosophila as a means to produce gynandromorphs for developmental studies. We describe a method to construct ring-X chromosomes in Drosophila via I-CreI-mediated exchange in rDNA, and then rapidly diagnose the recovery of ring chromosomes via FLP-mediated sister chromatid exchange within the ring. The method we describe provides a ready means to tailor the genetic content of ring-X chromosomes, making it suited to produce ring-X chromosomes for a variety of experimental purposes.
摘要
环形染色体对于研究其在减数分裂和有丝分裂中行为的遗传学家和细胞生物学家来说具有根本的研究意义。此外，与某些X环形染色体相关的有丝分裂不稳定性已被证明在果蝇中是有用的，可作为一种手段来产生用于发育研究的雌雄嵌合体。我们描述了一种通过rDNA中I-CreI介导的交换在果蝇中构建X环形染色体的方法，然后通过环内FLP介导的姐妹染色单体交换快速诊断环形染色体的回收情况。我们所描述的方法提供了一种现成的手段来定制X环形染色体的遗传内容，使其适合于为各种实验目的产生X环形染色体。
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