Department of Biology, Washington University in St. Louis, St. Louis, MO 63130-4899, USA.
Photosynth Res. 2010 Dec;106(3):227-38. doi: 10.1007/s11120-010-9598-9. Epub 2010 Nov 18.
Ten naturally occurring chlorophylls (a, b, c (2), d) and bacteriochlorophylls (a, b, c, d, e, g) were purified and studied using the optical spectroscopic techniques of both steady state and time-resolved absorption and fluorescence. The studies were carried out at room temperature in nucleophilic solvents in which the central Mg is hexacoordinated. The comprehensive studies of singlet excited state lifetimes show a clear dependency on the structural features of the macrocycle and terminal substituents. The wide-ranging studies of triplet state lifetime demonstrate the existence of an energy gap law for these molecules. The knowledge of the dynamics and the energies of the triplet state that were obtained in other studies allowed us to construct an energy gap law expression that can be used to estimate the triplet state energies of any (B)chlorophyll molecule from its triplet lifetime obtained in a liquid environment.
十种天然存在的叶绿素（a、b、c（2）、d）和细菌叶绿素（a、b、c、d、e、g）被提纯并使用稳态和时间分辨吸收和荧光的光学光谱技术进行了研究。这些研究在室温下在亲核溶剂中进行，其中中心 Mg 是六配位的。单重激发态寿命的综合研究表明，其对大环和末端取代基的结构特征有明显的依赖性。三重态寿命的广泛研究证明了这些分子存在能隙定律。在其他研究中获得的三重态动力学和能量知识使我们能够构建一个能隙定律表达式，该表达式可用于根据在液体环境中获得的三重态寿命估算任何（B）叶绿素分子的三重态能量。
