Institut für Biophysikalische Chemie, Medizinische Hochschule Hannover, Carl-Neuberg-Straße, Germany.
Cell Mol Life Sci. 2011 Aug;68(16):2751-67. doi: 10.1007/s00018-010-0590-5. Epub 2010 Nov 18.
Dictyostelium discoideum cells produce five dynamin family proteins. Here, we show that dynamin B is the only member of this group of proteins that is initially produced as a preprotein and requires processing by mitochondrial proteases for formation of the mature protein. Our results show that dynamin B-depletion affects many aspects of cell motility, cell-cell and cell-surface adhesion, resistance to osmotic shock, and fatty acid metabolism. The mature form of dynamin B mediates a wide range and unique combination of functions. Dynamin B affects events at the plasma membrane, peroxisomes, the contractile vacuole system, components of the actin-based cytoskeleton, and cell adhesion sites. The modulating effect of dynamin B on the activity of the contractile vacuole system is unique for the Dictyostelium system. Other functions displayed by dynamin B are commonly associated with either classical dynamins or dynamin-related proteins.
粘菌细胞产生五种胞质动力蛋白家族蛋白。在这里，我们表明，动力蛋白 B 是该蛋白组中唯一最初作为前体蛋白产生的成员，需要线粒体蛋白酶加工才能形成成熟蛋白。我们的结果表明，动力蛋白 B 耗竭会影响细胞运动、细胞-细胞和细胞表面黏附、抗渗透压冲击以及脂肪酸代谢的许多方面。成熟形式的动力蛋白 B 介导广泛而独特的功能组合。动力蛋白 B 影响质膜、过氧化物酶体、收缩液泡系统、肌动蛋白细胞骨架成分和细胞黏附位点的事件。动力蛋白 B 对收缩液泡系统活性的调节作用是粘菌系统所特有的。动力蛋白 B 表现出的其他功能通常与经典动力蛋白或与动力蛋白相关蛋白相关联。
