Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, South Korea.
Phytother Res. 2011 Jun;25(6):809-15. doi: 10.1002/ptr.3337. Epub 2010 Nov 17.
A large aquatic herb, Nelumbo nucifera Gaertn, has psychopharmacological effects similar to minor tranquillizers and antistress agents. This study examined the effects of Nelumbo nucifera rhizome extracts (NRE) on cell proliferation and neuroblast differentiation in the hippocampal dentate gyrus (DG) of a rat model of scopolamine-induced amnesia. Immunohistochemical markers included Ki67, an endogenous marker for active cell cycle, and doublecortin (DCX), a marker for immature neurons and migratory neuroblasts. Scopolamine was administered for 28 days via an ALzet minipump (44 mg/mL delivered at 2.5 µL/h). NRE was administered by gavage, 1 g/kg per day for 28 days. The administration of scopolamine significantly reduced the number of Ki67- and DCX-immunoreactive cells in the DG, whereas scopolamine did not induce any significant changes in mature neurons in the DG. The administration of NRE significantly ameliorated the scopolamine-induced reduction of Ki67- and DCX-immunoreactive cells in the DG. In addition, the administration of NRE significantly restored the scopolamine-induced reduction of brain-derived neurotrophic factor in DG homogenates. These results suggest that NRE can ameliorate the scopolamine-induced reductions of cell proliferation, neuroblast differentiation and BDNF levels.
大型水生草本植物芡，具有类似镇静剂和抗应激剂的精神药理学作用。本研究探讨了芡根茎提取物（NRE）对东莨菪碱诱导遗忘大鼠模型海马齿状回（DG）细胞增殖和神经母细胞分化的影响。免疫组织化学标志物包括 Ki67，这是细胞周期活跃的内源性标志物，以及双皮质素（DCX），这是不成熟神经元和迁移性神经母细胞的标志物。东莨菪碱通过 Alzet 微型渗透泵（44mg/mL，以 2.5µL/h 的速度输送）连续 28 天给药。NRE 通过灌胃给药，每天 1g/kg，连续 28 天。东莨菪碱给药显著减少了 DG 中 Ki67 和 DCX 免疫反应性细胞的数量，而东莨菪碱对 DG 中的成熟神经元没有引起任何显著变化。NRE 的给药显著改善了东莨菪碱诱导的 DG 中 Ki67 和 DCX 免疫反应性细胞减少。此外，NRE 的给药显著恢复了东莨菪碱诱导的 DG 脑源性神经营养因子减少。这些结果表明，NRE 可以改善东莨菪碱诱导的细胞增殖、神经母细胞分化和 BDNF 水平降低。
