Chowdhury S K, Katta V, Chait B T
Rockefeller University, New York, NY 10021.
Biochem Biophys Res Commun. 1990 Mar 16;167(2):686-92. doi: 10.1016/0006-291x(90)92080-j.
Electrospray ionization mass spectrometric peptide mapping is demonstrated to be a useful new technique for protein structure analysis. The procedure involves the digestion of the protein with trypsin and subsequent analysis of the total unfractionated digest by electrospray ionization mass spectrometry. The utility of the technique for investigating protein structure is illustrated by a peptide mapping analysis of human apolipoprotein AI (Mr = 28 kDa). The technique is rapid, sensitive, and requires no prior separation of the peptides. The discrimination effects observed in other mass spectrometric methods are less important in the present procedure.
电喷雾电离质谱肽图分析被证明是一种用于蛋白质结构分析的有用新技术。该过程包括用胰蛋白酶消化蛋白质，随后通过电喷雾电离质谱对未分级的总消化物进行分析。通过对人载脂蛋白AI（Mr = 28 kDa）的肽图分析说明了该技术在研究蛋白质结构方面的实用性。该技术快速、灵敏，且无需预先分离肽段。在本过程中，其他质谱方法中观察到的歧视效应不太重要。
