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Direct peptide mapping of sickle-cell hemoglobin using electrospray mass spectrometry.

作者信息

Sakairi M

机构信息

Central Research Laboratory, Hitachi Ltd., Tokyo, Japan.

出版信息

Rapid Commun Mass Spectrom. 1993 Dec;7(12):1108-12. doi: 10.1002/rcm.1290071210.

DOI:10.1002/rcm.1290071210
PMID:8280919
Abstract

Direct peptide mapping of sickle-cell hemoglobin (Hb S) using electrospray mass spectrometry is demonstrated to be a rapid, sensitive and useful technique for providing information concerning the location of variation. By fully comparing electrospray mass spectra of digests of sickle-cell and normal hemoglobins, using L-1-tosylamide-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin, the point mutation of B1 fragment of beta-chain in Hb S can be confirmed, taking into consideration that all the other fragments are completely identical in the two proteins.

摘要

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