Mandal Vivekananda, Sen Sukanta Kumar, Mandal Narayan Chandra
P.G. Department of Botany, Darjeeling Govt. College, Darjeeling 734 101, India.
Indian J Biochem Biophys. 2008 Apr;45(2):106-10.
A strain of Pediococcus acidilactici LAB 5 was isolated from vacuum-packed fermented meat product, in order to obtain a novel bacteriocin from food-grade organisms. Optimized culture conditions for bacteriocin production in different media (viz., MRS, TGE, TGE + buffer, TGE + Tween 80, and TGE + Tween 80 + buffer) and at different temperatures and pH conditions were reported. TGE + Tween 80 + buffer medium was found to be most effective for bacteriocin production (about 2400 AU/ml) by this strain, when incubated at 37 degrees C for 24 h. Bacteriocin, partially purified by adsorption-desorption method showed molecular mass of 10.3 kDa and produced prominent inhibition zone in activity gel. It showed significant storage stability both at high as well as in low temperatures for up to 6 months and retained its activity in a number of organic solvents, except in 2-mercaptoethanol. The treatment with amylase and lysozyme did not change its activity, but it lost its activity on proteinase K treatment. Antibacterial efficacy of bacteriocin was proved against some food spoilage and human pathogenic bacteria like Enterococcus, Leuconostoc, Listeria, Staphylococcus and Streptococcus.
从真空包装的发酵肉制品中分离出一株嗜酸乳杆菌LAB 5，目的是从食品级微生物中获得一种新型细菌素。报道了在不同培养基（即MRS、TGE、TGE + 缓冲液、TGE + 吐温80以及TGE + 吐温80 + 缓冲液）中以及在不同温度和pH条件下生产细菌素的优化培养条件。发现当在37℃孵育24小时时，TGE + 吐温80 + 缓冲液培养基对该菌株产生细菌素最为有效（约2400 AU/ml）。通过吸附 - 解吸法部分纯化的细菌素显示分子量为10.3 kDa，并在活性凝胶中产生明显的抑菌圈。它在高温和低温下均表现出显著的储存稳定性，长达6个月，并且在多种有机溶剂中都能保持其活性，但在2 - 巯基乙醇中除外。用淀粉酶和溶菌酶处理不会改变其活性，但用蛋白酶K处理会使其失去活性。已证明该细菌素对一些食品腐败菌和人类病原菌如肠球菌、明串珠菌、李斯特菌、葡萄球菌和链球菌具有抗菌功效。
