Bramley T A, Menzies G S, Williams R J, Adams D J, Kinsman O S
University of Edinburgh, Department of Obstetrics and Gynaecology, Scotland, U.K.
Biochem Biophys Res Commun. 1990 Mar 30;167(3):1050-6. doi: 10.1016/0006-291x(90)90629-2.
The presence of specific binding sites for [125I]-labelled hLH and hCG is described in Candida species. Binding was present in three strains of Candida albicans, and in Candida tropicalis, and was greatest in microsomes, though binding was also present in cytosol fractions. hLH and hCG mutually competed for these binding sites. Other hormones did not bind and did not compete for hLH binding sites. Scatchard plots showed two classes of binding sites, one with high affinity, low capacity and the other with lower affinity, high capacity binding in both microsomes and cytosol. This is the first report of specific binding sites for mammalian peptide hormones in a yeast.
已在念珠菌属中发现了[125I]标记的人促黄体生成素（hLH）和人绒毛膜促性腺激素（hCG）的特异性结合位点。在白色念珠菌的三个菌株以及热带念珠菌中均存在结合现象，且在微粒体中的结合最强，不过在胞质溶胶组分中也存在结合。hLH和hCG相互竞争这些结合位点。其他激素不结合也不竞争hLH结合位点。Scatchard图显示有两类结合位点，一类具有高亲和力、低容量，另一类在微粒体和胞质溶胶中均具有较低亲和力、高容量结合。这是酵母中存在哺乳动物肽类激素特异性结合位点的首次报道。
