Murri M, Garcia-Delgado R, Alcázar-Ramirez J, Linde F, Fernández-Ramos A, Cardona F, Tinahones F J
Servicio de Endocrinología y Nutrición, Hospital Clínico Universitario Virgen de la Victoria, 29010 Málaga, Spain.
Clin Lab. 2010;56(9-10):397-406.
Several indirect techniques have been used for measuring oxidative stress in sleep apnoea-hypopnoea syndrome (SAHS) patients. The purpose of this study was to find out if both, cellular or plasma oxidative stress evaluations, are good estimators to assess oxidative stress in SAHS patients before and after one month's CPAP treatment.
The study included 28 SAHS patients requiring CPAP treatment and 15 healthy control subjects. Plasma and serum oxidative stress biomarkers (lipid peroxidation, total antioxidant capacity, and the activities of glutathione peroxidase, glutathione reductase, glutathione s-transferase, catalase and superoxide dismutase) were measured using commercial kits. Cellular oxidative stress biomarkers (mitochondrial membrane potential, intracellular glutathione, superoxide anion, and hydrogen peroxide) were analysed by flow cytometry. The Wilcoxon test for paired samples was used to compare oxidative stress and clinical parameters in patients before and after treatment with CPAP. Relationships in oxidative stress markers between controls and patients were analyzed using the Mann-Whitney U test. The Spearman correlation coefficient was calculated to estimate the linear correlations between variables.
Oxidative stress was notably decreased after CPAP. Before CPAP, SAHS severity positively correlated with hydrogen peroxide levels, while negative correlations were observed between SAHS severity and plasma TAC in patients. Also, plasma glutathione peroxidase activity negatively correlated with cellular superoxide anion, while plasma superoxide dismutase activity positively correlated with intracellular glutathione. After CPAP, plasma TAC and glutathione peroxidase activity negatively correlated with cellular hydrogen peroxide and superoxide anion.
In conclusion, this study seems to confirm that plasma and cellular assessment reflect, in the same way, the oxidative stress status of the studied patients. Furthermore, plasma total antioxidant capacity as well as cellular hydrogen peroxide levels can be good markers for assessing oxidative stress in SAHS patients.
已经使用了几种间接技术来测量睡眠呼吸暂停低通气综合征（SAHS）患者的氧化应激。本研究的目的是确定细胞或血浆氧化应激评估是否都是评估SAHS患者在持续气道正压通气（CPAP）治疗前后氧化应激的良好指标。
该研究纳入了28名需要CPAP治疗的SAHS患者和15名健康对照者。使用商业试剂盒测量血浆和血清氧化应激生物标志物（脂质过氧化、总抗氧化能力以及谷胱甘肽过氧化物酶、谷胱甘肽还原酶、谷胱甘肽S-转移酶、过氧化氢酶和超氧化物歧化酶的活性）。通过流式细胞术分析细胞氧化应激生物标志物（线粒体膜电位、细胞内谷胱甘肽、超氧阴离子和过氧化氢）。使用配对样本的Wilcoxon检验比较CPAP治疗前后患者的氧化应激和临床参数。使用Mann-Whitney U检验分析对照组和患者之间氧化应激标志物的关系。计算Spearman相关系数以估计变量之间的线性相关性。
CPAP治疗后氧化应激显著降低。在CPAP治疗前，SAHS严重程度与过氧化氢水平呈正相关，而患者的SAHS严重程度与血浆总抗氧化能力呈负相关。此外，血浆谷胱甘肽过氧化物酶活性与细胞超氧阴离子呈负相关，而血浆超氧化物歧化酶活性与细胞内谷胱甘肽呈正相关。CPAP治疗后，血浆总抗氧化能力和谷胱甘肽过氧化物酶活性与细胞过氧化氢和超氧阴离子呈负相关。
总之，本研究似乎证实血浆和细胞评估以相同方式反映了所研究患者的氧化应激状态。此外，血浆总抗氧化能力以及细胞过氧化氢水平可能是评估SAHS患者氧化应激的良好标志物。
