Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, 5 Research Court, Rockville, MD 20850, USA.
Exp Hematol. 2011 Feb;39(2):142-50. doi: 10.1016/j.exphem.2010.11.004. Epub 2010 Nov 16.
To better define the nature of the transient neutropenia shortly following granulocyte-colony stimulating factor (G-CSF) administration.
To evaluate the disappearance of neutrophils, we investigated neutrophil trafficking. Ratios of neutrophil number to background cellularity for C57BL/6 LysM-EGFP knock-in mice and rhesus macaques were determined in the lung, liver, spleen, and kidney after G-CSF administration.
For the C57BL/6 LysM-EGFP knock-in mice, the enhanced green fluorescent protein expression (EGFP(+)) cells increased in the lung and spleen within 15 minutes of administering 50 μg/kg G-CSF subcutaneously, and continued to increase in the lung and spleen from 15 minutes to 30 minutes. At 240 minutes, the pulmonary infiltrate declined to a level comparable to the level at 15 minutes, while in the spleen EGFP(+) cells continued to increase. For rhesus macaques, CD18(+) cells also significantly increased in the lung 30 minutes after administration of 10 μg/kg G-CSF subcutaneously compared to the control level.
These results suggest that the transient neutropenia following G-CSF administration in the mouse and nonhuman primate is associated with an accumulation of neutrophils within pulmonary and splenic vasculature.
更好地定义粒细胞集落刺激因子（G-CSF）给药后短暂性中性粒细胞减少的性质。
为了评估中性粒细胞的消失，我们研究了中性粒细胞的迁移。在给予 G-CSF 后，我们在 C57BL/6 LysM-EGFP 嵌合小鼠和恒河猴的肺、肝、脾和肾中测定中性粒细胞数量与背景细胞比。
对于 C57BL/6 LysM-EGFP 嵌合小鼠，在给予 50μg/kg G-CSF 皮下后 15 分钟内，肺和脾中增强型绿色荧光蛋白表达（EGFP(+)）细胞增加，并在肺和脾中从 15 分钟增加到 30 分钟。240 分钟时，肺部浸润物下降至与 15 分钟时相当的水平，而在脾中 EGFP(+)细胞继续增加。对于恒河猴，与对照组相比，在给予 10μg/kg G-CSF 皮下后 30 分钟，肺中 CD18(+)细胞也显著增加。
这些结果表明，在小鼠和非人类灵长类动物中，G-CSF 给药后短暂性中性粒细胞减少与肺和脾血管内中性粒细胞的积累有关。
