Hattori K, Orita T, Oheda M, Tamura M, Ono M
Fuji-Gotemba Research Laboratories, Chugai Pharmaceutical Co., Ltd., Shizuoka, Japan.
In Vivo. 1996 May-Jun;10(3):319-27.
To examine the differential in vivo effects of granulocyte colony-stimulating factor (G-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) on the recovery from neutropenia caused by chemotherapy, we directly compared the actions of these CSFs on neutrophil count in the bone marrow, spleen, and peripheral blood of mice treated with an anticancer drug, cyclophosphamide (CPA). Both G-CSF and GM-CSF stimulated the generation of neutrophils in bone marrow, but GM-CSF is only about 1/20 as effective as G-CSF. Both CSFs also stimulated the release of the generated neutrophils therefrom. G-CSF mobilized the neutrophils into both the circulation pool and the marginal pool, and these neutrophils were able to migrate into the inflammatory site when stimulated by casein. On the other hand, GM-CSF mobilize the neutrophils into the marginal pool but not the circulation pool, and they were not able to migrate into the inflammatory site. These findings indicate that G-CSF physiologically plays an important role as a factor which stimulates neutrophil mobilization from bone marrow into circulation as well as neutrophil generation in the bone marrow.
为了研究粒细胞集落刺激因子（G-CSF）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）对化疗所致中性粒细胞减少恢复的体内差异作用，我们直接比较了这些集落刺激因子对用抗癌药物环磷酰胺（CPA）治疗的小鼠骨髓、脾脏和外周血中中性粒细胞计数的影响。G-CSF和GM-CSF均刺激骨髓中中性粒细胞的生成，但GM-CSF的效力仅约为G-CSF的1/20。两种集落刺激因子也都刺激生成的中性粒细胞从骨髓中释放。G-CSF将中性粒细胞动员到循环池和边缘池中，并且当受到酪蛋白刺激时，这些中性粒细胞能够迁移到炎症部位。另一方面，GM-CSF将中性粒细胞动员到边缘池中，但不能动员到循环池中，并且它们不能迁移到炎症部位。这些发现表明，G-CSF在生理上作为一种刺激中性粒细胞从骨髓动员到循环以及骨髓中中性粒细胞生成的因子发挥重要作用。
