School of Life Science, East China Normal University, North Zhong-Shan Road, Shanghai, China.
Fish Shellfish Immunol. 2011 Jan;30(1):324-9. doi: 10.1016/j.fsi.2010.11.008. Epub 2010 Nov 16.
Macrophage migration inhibitory factor (MIF) as a multi-functional cytokine mediating both innate and adaptive immune responses, however, their function within the innate immune system of invertebrates remains largely unknown. Therefore, we investigated the immune functionality of MIF in Chinese mitten crab (Eriocheir sinensis), a commercially important and disease vulnerable aquaculture species. The full-length MIF cDNA (704 bp) was cloned via PCR based upon an initial expressed sequence tag (EST) isolated from a E. sinensis cDNA library. The MIF cDNA contained a 363 bp open reading frame (ORF) that encoded a putative 120 amino acid (aa) protein. Comparisons with other reported invertebrate and vertebrate MIF sequences revealed conserved enzyme active sites. MIF mRNA expression in E. sinensis was (a) tissue-specific, with the highest expression observed in hepatotpancreas, and (b) responsive in hemocytes, hepatopancreas and gill to a Vibrio anguillarum challenge, with peak exposure observed 8 h, 12 h and 12 h post-injection, respectively. Collectively, data demonstrate the successful isolation of MIF from the Chinese mitten crab, and its involvement in the innate immune system of an invertebrate.
巨噬细胞移动抑制因子（MIF）作为一种多功能细胞因子，介导先天和适应性免疫反应，然而，其在无脊椎动物先天免疫系统中的功能在很大程度上尚不清楚。因此，我们研究了 MIF 在中华绒螯蟹（Eriocheir sinensis）中的免疫功能，中华绒螯蟹是一种具有商业重要性和易患病的水产养殖物种。通过基于从中华绒螯蟹 cDNA 文库中分离的初始表达序列标签（EST）的 PCR，克隆了全长 MIF cDNA（704 bp）。MIF cDNA 包含一个 363 bp 的开放阅读框（ORF），编码一个假定的 120 个氨基酸（aa）蛋白。与其他报道的无脊椎动物和脊椎动物 MIF 序列的比较显示出保守的酶活性位点。MIF 在中华绒螯蟹中的 mRNA 表达具有（a）组织特异性，在肝胰腺中表达最高，（b）对鳗弧菌刺激的血细胞、肝胰腺和鳃有反应，分别在注射后 8 h、12 h 和 12 h 达到峰值。总之，数据表明成功地从中华绒螯蟹中分离出 MIF，并证明其参与了无脊椎动物的先天免疫系统。
