School of Life Science, East China Normal University, North Zhongshan Road, 3663 Shanghai, China.
Dev Comp Immunol. 2010 Nov;34(11):1170-4. doi: 10.1016/j.dci.2010.06.011. Epub 2010 Jun 26.
Cathepsins, a superfamily of hydrolytic enzymes produced and enclosed within lysosomes, function in immune response in vertebrates; however, their function within the innate immune system of invertebrates remains largely unknown. Therefore, we investigated the immune functionality of cathepsin C (catC) in Chinese mitten crab (Eriocheir sinensis), a commercially important and disease vulnerable aquaculture species. The full-length catC cDNA (1481 bp) was cloned via PCR based upon an initial expressed sequence tag (EST) isolated from a hepatopancreatic cDNA library. The catC cDNA contained a 1284 bp open reading frame (ORF) that encoded a putative 427 amino acid (aa) protein. Comparisons with other reported invertebrate and vertebrate cathepsins sequences revealed high percent identity. CatC mRNA expression in E. sinensis was responsive in hemocytes to a Vibrio anguillarum challenge, with peak exposure observed 6 h post-injection. Collectively, data demonstrate the successful isolation of catC from the Chinese mitten crab, and its involvement in the innate immune system of an invertebrate.
组织蛋白酶是一个水解酶的超家族，在脊椎动物的免疫反应中产生并包含在溶酶体中；然而，它们在无脊椎动物的固有免疫系统中的功能在很大程度上仍然未知。因此，我们研究了中国绒螯蟹（Eriocheir sinensis）组织蛋白酶 C（catC）的免疫功能，中国绒螯蟹是一种具有商业重要性和易患病的水产养殖物种。通过基于从肝胰腺 cDNA 文库中分离的初始表达序列标签（EST）的 PCR，克隆了全长 catC cDNA（1481 bp）。catC cDNA 包含一个 1284 bp 的开放阅读框（ORF），编码一个假定的 427 个氨基酸（aa）的蛋白质。与其他报道的无脊椎动物和脊椎动物组织蛋白酶序列的比较显示出高百分比的同一性。在中国绒螯蟹的血细胞中，catC mRNA 的表达对鳗弧菌的刺激有反应，在注射后 6 小时观察到峰值暴露。总的来说，数据表明成功地从中国绒螯蟹中分离出 catC，并证明其参与了无脊椎动物的固有免疫系统。
