Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, United States.
Eur J Cell Biol. 2011 Jan;90(1):37-48. doi: 10.1016/j.ejcb.2010.09.013. Epub 2010 Nov 18.
Cell spreading is a critical component of numerous physiological phenomena including cancer metastasis, embryonic development, and mitosis. We have previously illustrated that cellular blebs appear after abrupt cell-substrate detachment and play a critical role in regulating membrane tension; however, the dynamics of bleb-substrate interactions during spreading remains unclear. Here we explore the role of blebs during endothelial cell spreading using chemical and osmotic modifications to either induce or inhibit bleb formation. We track cell-substrate dynamics as well as individual blebs using surface sensitive microscopic techniques. Blebbing cells (both control and chemically induced) exhibit increased lag times prior to fast growth. Interestingly, lamellae appear later for blebbing compared to non-blebbing cells, and in all cases, lamellae signal the start of fast spreading. Our results indicate that cellular blebs play a key role in the early stage of cell spreading, first by controling the initial cell adhesion and then by presenting a dynamic inhibition of cell spreading until a lamella appears and fast spreading ensues.
细胞铺展是许多生理现象的关键组成部分，包括癌症转移、胚胎发育和有丝分裂。我们之前已经表明，细胞泡囊在突然的细胞-基底物脱离后出现，并在调节膜张力方面发挥关键作用；然而，在铺展过程中泡囊-基底物相互作用的动力学仍不清楚。在这里，我们使用化学和渗透修饰来诱导或抑制泡囊形成，来探索泡囊在血管内皮细胞铺展过程中的作用。我们使用表面敏感的显微镜技术跟踪细胞-基底物动力学和单个泡囊。起泡的细胞（包括对照和化学诱导的细胞）在快速生长之前表现出增加的滞后时间。有趣的是，与非起泡细胞相比，泡囊细胞的片状伪足出现得更晚，而且在所有情况下，片状伪足都标志着快速铺展的开始。我们的结果表明，细胞泡囊在细胞铺展的早期阶段发挥关键作用，首先控制初始细胞黏附，然后通过动态抑制细胞铺展，直到出现片状伪足并开始快速铺展。
