Schmutzler Sandra, Bachmann Lisa, Fuhrmann Herbert, Schumann Julia
Institute of Physiological Chemistry, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 1 04103 Leipzig, Germany.
Acta Vet Hung. 2010 Dec;58(4):453-64. doi: 10.1556/AVet.58.2010.4.6.
Mast cells play a key role in the immune response. Thereby, the balance of oxidative metabolism is of importance in mast cell mediator synthesis and release. Fatty acids may modify mast cell function in several ways. In this study, we investigated the influence of polyunsaturated fatty acids (PUFAs) on oxidative parameters of a canine mastocytoma cell line. C2 cells were cultured in media supplemented with linoleic acid, arachidonic acid, alpha-linolenic acid and eicosapentaenoic acid, respectively. Production of reactive oxygen species (ROS) as well as lipid peroxides was tested. Furthermore, stressor-induced DNA damage was measured. Exposure of the cells to PUFAs resulted in a significant increase in the synthesis of both ROS and lipid peroxides. Distinct differences between the PUFAs tested underline the impact of the unsaturation degree of fatty acids as well as the position of double bonds on mast cells.
肥大细胞在免疫反应中起关键作用。因此，氧化代谢平衡在肥大细胞介质的合成和释放中至关重要。脂肪酸可能通过多种方式改变肥大细胞功能。在本研究中，我们调查了多不饱和脂肪酸（PUFAs）对犬肥大细胞瘤细胞系氧化参数的影响。分别在补充了亚油酸、花生四烯酸、α-亚麻酸和二十碳五烯酸的培养基中培养C2细胞。检测了活性氧（ROS）以及脂质过氧化物的产生。此外，还测量了应激源诱导的DNA损伤。细胞暴露于PUFAs导致ROS和脂质过氧化物的合成显著增加。所测试的PUFAs之间的明显差异突显了脂肪酸不饱和度以及双键位置对肥大细胞的影响。
