Asayama J, Tatsumi T, Miyazaki H, Omori I, Shirayama T, Inoue D, Nakagawa M
Second Department of Medicine, Kyoto Prefectural University of Medicine, Japan.
Eur J Pharmacol. 1990 Jan 17;175(3):309-15. doi: 10.1016/0014-2999(90)90569-r.
We used saponin-treated skinned fibers isolated from papillary muscles of the right ventricle of guinea pigs to compare the effects of diltiazem on the spontaneous cyclic Ca2+ release from the sarcoplasmic reticulum (SR) of cardiac muscle with the effects of caffeine. Both diltiazem and caffeine increased the frequency of cyclic contractions in EGTA (ethyleneglycol-bis-(beta-aminoethylether) N,N,N',N'-tetra-acetate)-free solution and induced an incomplete, concentration-dependent relaxation of the muscle. The results suggest that the spectrum of the pharmacological effects of diltiazem on the SR is similar to that for caffeine, that is diltiazem accelerates the spontaneous Ca2+ release from the SR and suppresses Ca2+ reuptake by the SR, and that diltiazem as well as caffeine may act on the SR in vivo in a specific condition.
我们使用从豚鼠右心室乳头肌分离得到的经皂角苷处理的去皮肤纤维，比较地尔硫䓬对心肌肌浆网（SR）自发钙循环释放的影响与咖啡因的影响。在无乙二醇双（β-氨基乙基醚）N，N，N'，N'-四乙酸（EGTA）的溶液中，地尔硫䓬和咖啡因均增加了周期性收缩的频率，并引起肌肉不完全的、浓度依赖性的舒张。结果表明，地尔硫䓬对肌浆网的药理作用谱与咖啡因相似，即地尔硫䓬加速肌浆网的自发钙释放并抑制肌浆网对钙的再摄取，并且地尔硫䓬以及咖啡因可能在特定条件下在体内作用于肌浆网。
