Crowley W R, O'Donohue T L, Wachslicht H, Jacobowitz D M
Brain Res. 1978 Oct 13;154(2):345-57. doi: 10.1016/0006-8993(78)90705-9.
Estradiol benzoate (EB) was administered, either alone or followed 48 h later by progesterone to ovariectomized rats. Plasma gonadotropins (FSH and LH) and steady state levels of norepinephrine (NE) and dopamine (DA) in 17 individual brain nuclei were assayed. In addition, catecholamines were measured after administration of the synthesis inhibitor alpha-methyltyrosine (alpha-MT) in order to assess hormonal influences on turnover. Treatment with EB, which lowered plasma FSH and LH, reduced the depletion of NE produced by alpha-MT in the lateral septum, interstitial nucleus of the stria terminalis, and central gray catecholamine area, and reduced the depletion of DA in the nucleus of the tractus diagonalis. EB enhanced NE depletion in the periventricular and anterior hypothalamic nuclei, and raised steady state levels of NE in the medial amygdaloid nucleus. These effects were reversed by subsequent treatment with progesterone, which stimulated FSH and LH release. EB plus progesterone enhanced the alpha-MT-induced depletion of NE over that observed with EB alone in the arcuate nucleus, and similarly enhanced DA depletion in the interstitial nucleus of the stria terminalis. EB plus progesterone prevented the depletion of NE by alpha-MT in the paraventricular and ventromedial nuclei, and also lowered resting NE levels in the paraventricular nucleus. The results suggest that catecholamine neurons in several discrete brain regions participate in the stimulatory and inhibitory feedback effects of ovarian hormones on gonadotropin secretion, and perhaps also on the hormonal induction of sexual receptivity.
对去卵巢大鼠单独给予苯甲酸雌二醇（EB），或在48小时后给予孕酮。检测了17个脑核中血浆促性腺激素（FSH和LH）以及去甲肾上腺素（NE）和多巴胺（DA）的稳态水平。此外，在给予合成抑制剂α-甲基酪氨酸（α-MT）后测量了儿茶酚胺，以评估激素对其周转的影响。用EB治疗可降低血浆FSH和LH水平，减少α-MT在外侧隔、终纹间质核和中央灰质儿茶酚胺区所导致的NE耗竭，并减少斜角带核中的DA耗竭。EB增强了室周核和下丘脑前核中的NE耗竭，并提高了杏仁内侧核中NE的稳态水平。随后用刺激FSH和LH释放的孕酮治疗可逆转这些作用。与单独使用EB相比，EB加孕酮增强了弓状核中α-MT诱导的NE耗竭，同样增强了终纹间质核中的DA耗竭。EB加孕酮可防止α-MT在室旁核和腹内侧核中导致的NE耗竭，还降低了室旁核中的静息NE水平。结果表明，几个离散脑区的儿茶酚胺神经元参与了卵巢激素对促性腺激素分泌的刺激和抑制反馈作用，也许还参与了激素诱导的性接受能力。
