Renner K J, Krey L C, Luine V N
Laboratory of Neuroendocrinology, Rockefeller University, New York, NY 10021.
Brain Res Bull. 1987 Aug;19(2):195-202. doi: 10.1016/0361-9230(87)90085-2.
The effect of progesterone (P) on monoamine levels and turnover was evaluated in 8 brain nuclei in estrogen-primed rats. Animals were subcutaneously (SC) injected with P or vehicle 21 hours after SC treatment with 5 micrograms of estradiol benzoate (EB). EB-primed animals treated with P showed high levels of lordosis behavior and an LH surge three hours later. Initial concentrations of norepinephrine (NE), dopamine (DA), serotonin (5HT) and 5-hydroxyindole acetic acid were determined in EB-saline treated controls 3 hours after P or vehicle. NE and DA turnover was estimated from the exponential decline of these amines 2 hours after IP injection of alpha-methyl-p-tyrosine (5 hours after P or vehicle). The accumulation of 5HT 20 min following IP injection of pargyline was used as an index of 5HT turnover. P did not affect the initial NE, 5HT or 5HIAA concentrations in any of the brain nuclei studied, but decreased DA content in the arcuate-median eminence region (Ar-ME). The DA rate constant was elevated in the nucleus of the diagonal band of Broca and the DA turnover rate was decreased in the Ar-ME. In the periventricular region (PVE, anterior hypothalamic level) the NE turnover rate (K, pg/microgram protein/hr) and rate constant (k, hr-1) decreased following P treatment. Progesterone treatment decreased the accumulation of 5HT in the ventromedial hypothalamus (VMN, pars lateralis) and the dorsal midbrain central grey (MCG). Progesterone effects on monoamine turnover were not found in the lateral septal, medial preoptic, anterior hypothalamic or dorsal raphe nuclei.(ABSTRACT TRUNCATED AT 250 WORDS)
在经雌激素预处理的大鼠的8个脑核中评估了孕酮（P）对单胺水平和周转率的影响。在用5微克苯甲酸雌二醇（EB）皮下处理21小时后，给动物皮下注射P或赋形剂。用P处理的经EB预处理的动物在3小时后表现出高频率的脊柱前凸行为和促黄体生成素激增。在注射P或赋形剂3小时后，测定经EB - 生理盐水处理的对照中去甲肾上腺素（NE）、多巴胺（DA）、5 - 羟色胺（5HT）和5 - 羟吲哚乙酸的初始浓度。在腹腔注射α - 甲基 - p - 酪氨酸2小时后（注射P或赋形剂5小时后），根据这些胺类的指数下降来估计NE和DA的周转率。腹腔注射优降宁20分钟后5HT的积累用作5HT周转率的指标。P对所研究的任何脑核中的初始NE、5HT或5 - 羟吲哚乙酸浓度均无影响，但降低了弓状 - 正中隆起区域（Ar - ME）中的DA含量。在布罗卡斜角带核中DA速率常数升高，而在Ar - ME中DA周转率降低。在室周区域（PVE，下丘脑前部水平），P处理后NE周转率（K，pg/微克蛋白质/小时）和速率常数（k，小时-1）降低。孕酮处理降低了腹内侧下丘脑（VMN，外侧部）和中脑背侧中央灰质（MCG）中5HT的积累。在外侧隔核、内侧视前核、下丘脑前部或背侧中缝核中未发现孕酮对单胺周转率的影响。（摘要截短为250字）
