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J Clin Microbiol. 2008 Aug;46(8):2780-2. doi: 10.1128/JCM.00557-08. Epub 2008 Jun 4.
2
Culture-based methods for detection and identification of Streptococcus agalactiae in pregnant women--what are we missing?基于培养的孕妇无乳链球菌检测与鉴定方法——我们遗漏了什么？
Eur J Clin Microbiol Infect Dis. 2008 Mar;27(3):241-3. doi: 10.1007/s10096-007-0421-2. Epub 2007 Nov 21.
3
Group B streptococcus prevalence in pregnant women from North-Eastern Italy: advantages of a screening strategy based on direct plating plus broth enrichment.意大利东北部孕妇中B族链球菌的流行情况：基于直接接种加肉汤增菌的筛查策略的优势
J Clin Pathol. 2007 Oct;60(10):1140-3. doi: 10.1136/jcp.2006.043489. Epub 2006 Dec 20.
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Diagn Microbiol Infect Dis. 2005 Jan;51(1):9-12. doi: 10.1016/j.diagmicrobio.2004.08.008.
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Comparison of two culture media and three sampling techniques for sensitive and rapid screening of vaginal colonization by group B streptococcus in pregnant women.两种培养基和三种采样技术用于孕妇B族链球菌阴道定植的敏感快速筛查的比较
J Clin Microbiol. 2004 Sep;42(9):3975-7. doi: 10.1128/JCM.42.9.3975-3977.2004.
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Use of direct latex agglutination testing of selective broth in the detection of group B strepptococcal carriage in pregnant women.使用选择性肉汤直接乳胶凝集试验检测孕妇B族链球菌携带情况。
Eur J Clin Microbiol Infect Dis. 2004 Jan;23(1):61-2. doi: 10.1007/s10096-003-1052-x. Epub 2003 Nov 6.
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J Clin Microbiol. 2002 Nov;40(11):4329-31. doi: 10.1128/JCM.40.11.4329-4331.2002.
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Prevention of perinatal group B streptococcal disease. Revised guidelines from CDC.预防围产期B族链球菌病。美国疾病控制与预防中心修订指南。
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Use of GBS media for rapid detection of group B streptococci in vaginal and rectal swabs from women in labor.使用GBS培养基快速检测分娩期妇女阴道和直肠拭子中的B族链球菌。
Eur J Clin Microbiol Infect Dis. 2001 Feb;20(2):120-2. doi: 10.1007/s100960000446.
10
Evaluation of the Granada agar plate for detection of vaginal and rectal group B streptococci in pregnant women.评估格拉纳达琼脂平板用于检测孕妇阴道和直肠B族链球菌的效果。
J Clin Microbiol. 1999 Aug;37(8):2648-51. doi: 10.1128/JCM.37.8.2648-2651.1999.

初步评估希钦斯-派克-托德-休伊特培养基（HPTH）用于检测孕妇中的 B 群链球菌。

Preliminary evaluation of Hitchens-Pike-Todd-Hewitt medium (HPTH) for detection of Group B Streptococci in pregnant women.

机构信息

Department of Medicine, State University of Maringá, Paraná, Brazil.

出版信息

J Clin Lab Anal. 2010;24(6):403-6. doi: 10.1002/jcla.20419.

DOI:10.1002/jcla.20419
PMID:21089171
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6647687/
Abstract

We aimed to compare Hitchens-Pike-Todd-Hewitt (HPTH) enrichment broth, Todd-Hewitt broth, and direct culture onto blood sheep agar containing selective antimicrobial (ABA) in screening Group B Streptococci (GBS) carriage in pregnant women. From April to June 2007, duplicate lower vaginal and duplicate anorectal specimens of 102 pregnant women at 35 weeks of gestation or more, who were assisted at Santa Casa de Misericórdia Hospital, Maringá, Paraná, Brazil, were screened by GBS using HPTH, Todd-Hewitt broth, and ABA. Twenty-five (24.5%) pregnant women had one or more positive culture for GBS in those media. The positive rate for each medium was 21.6% (22/102) in the HPTH, 12.8% (13/102) in ABA, and 11.8% (12/102) in Todd-Hewitt broth. HPTH seems to be a high satisfactory medium for screening GBS in vaginal and anorectal samples in pregnant women and additional studies would be interesting in a larger number of pregnant women and in different laboratories.

摘要

我们旨在比较希钦斯-派克-托德-休伊特（HPTH）增菌肉汤、托德-休伊特肉汤和直接在含有选择性抗菌剂的血绵羊琼脂上培养，以筛选孕妇携带 B 组链球菌（GBS）。2007 年 4 月至 6 月，对巴西巴拉那州马兰加市圣卡萨德米西库里亚医院的 102 名 35 周或以上妊娠的孕妇进行了阴道和直肠双份标本检测，使用 HPTH、托德-休伊特肉汤和 ABA 对 GBS 进行了筛查。在这些培养基中，有 25 名（24.5%）孕妇有一个或多个 GBS 阳性培养物。HPTH、ABA 和 Todd-Hewitt 肉汤的阳性率分别为 21.6%（22/102）、12.8%（13/102）和 11.8%（12/102）。HPTH 似乎是一种对孕妇阴道和直肠标本中 GBS 进行筛查的高满意度培养基，在更多孕妇和不同实验室中进行更多研究将很有趣。