Department of Clinical Laboratory, Peking University First Hospital, Beijing, China.
J Clin Lab Anal. 2010;24(6):418-21. doi: 10.1002/jcla.20422.
Serum separator tubes were introduced 35 years ago and were widely used in the clinical laboratory in China for routine collection of blood because of providing a closed system that allowed for collection, transport, processing, sampling, and storage of specimens. This type of tubes facilitated rapid separation of serum from cellular constituents of blood and also prevented hemolysis upon prolonged storage. However, there were some limitations associated with gel tubes (i.e., gel and analyte stability). In order to circumvent these problems, BD released a new serum separator tube containing a new gel (BD SST(™) II Plus). We investigated the performance of BD SST(™) II Plus tubes for tumor marker tests using BD Serum Glass tubes as controls. Equivalence between the BD SST(™) II Plus and BD Serum Glass tubes was demonstrated for all analytes at initial time. Also, all analytes remained stable when stored in BD SST(™) II Plus tubes up to 72 hr. Concentration of neuron-specific enolase tended to increase with preservation time up to 72 hr in BD Serum Glass tubes. We conclude that BD SST(™) II Plus was suitable for collection of blood and storage of serum for tumor marker tests.
血清分离管于 35 年前问世，由于提供了一个封闭系统，允许采集、运输、处理、采样和储存标本，因此在中国的临床实验室中被广泛用于常规采集血液。这种类型的试管便于血液的血清与细胞成分快速分离，并且在长时间储存时也能防止溶血。然而，凝胶管（即凝胶和分析物稳定性）存在一些局限性。为了规避这些问题，BD 推出了一种新的血清分离管，其中含有一种新的凝胶（BD SST(™) II Plus）。我们使用 BD 血清玻璃管作为对照，研究了 BD SST(™) II Plus 管在肿瘤标志物检测中的性能。在初始时间，BD SST(™) II Plus 管和 BD 血清玻璃管之间所有分析物的性能均具有等效性。此外，所有分析物在 BD SST(™) II Plus 管中储存长达 72 小时时均保持稳定。神经元特异性烯醇化酶的浓度在 BD 血清玻璃管中随着保存时间的延长至 72 小时而趋于增加。我们得出结论，BD SST(™) II Plus 适用于采集血液和储存用于肿瘤标志物检测的血清。
