Li Zhangqiu, Zhang Meixia, Hu Haiyan, Liu Shunhui, Lu Zhigang
Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2010 Oct;27(5):1146-51.
HLA-A * 0201, HLA-A * 1101, and HLA-A * 2401 CTL restricted epitopes of platelet membrane glycoprotein II b/III a antibody of human and mice were predicted by use of SYFPEITHI, RANKPEP, BIMAS, SVMHC, PREDEP, MHCPRED, and PROPRED predictive programs. In the results, the peptides (found in HLAPRED) that can lead to autoimmune disease and have been published were removed; and the epitopes of HLA-A * 0201 must cover the epitopes of HLA-A * 1101 and HLA-A * 2401 being combined to predTAP and TAPPred for predicting the binding affinity of peptides toward the TAP transporter and NetChop, MAPPP, PAProc for predicting cleavages; HLA-DR Th restricted epitopes of GPII b/III a antibody were predicted by SYFPEITHI, RANKPEP, MHCPRED, and HLAPRED, after removal of the peptides (found in HLAPRED) that can lead to autoimmune disease and have been published, the Th epitopes must cover the CTL mixed epitopes as being stated above. The secondary structure, hydrophobic regions, flexibility, surface probability and the B cell epitope were predicted by using various methods. Ten mixed peptides of T cell epitopes were selected from more than 1 740 peptides. They were located at the aa9-115, aa24-38, aa50-64, aa65-81, aa109-121 of anti-GP II b/III a-Human and the aal-15, aa26-40, aa46-60, aa68-82, aa93-107 of anti-GP II b/III a-Mice. B cell epitopes of anti-GP II b/III a-Human might locate at aa5-9, aa22-30, aa40-46, aa55-71, aa80-90, aa100-105, aa110-115; and the epitopes of anti-GP II b/III a-Human might locate at aa5-10, aa38-43, aa58-70, aa77-84, and aa99-105.
利用SYFPEITHI、RANKPEP、BIMAS、SVMHC、PREDEP、MHCPRED和PROPRED预测程序，预测了人和小鼠血小板膜糖蛋白IIb/IIIa抗体的HLA - A * 0201、HLA - A * 1101和HLA - A * 2401细胞毒性T淋巴细胞（CTL）限制性表位。结果中，去除了（在HLAPRED中发现的）已发表的可导致自身免疫性疾病的肽段；并且HLA - A * 0201的表位必须覆盖HLA - A * 1101和HLA - A * 2401的表位，将其与predTAP和TAPPred结合以预测肽段与TAP转运体的结合亲和力，并用NetChop、MAPPP、PAProc预测裂解情况；通过SYFPEITHI、RANKPEP、MHCPRED和HLAPRED预测GPIIb/IIIa抗体的HLA - DR辅助性T细胞（Th）限制性表位，去除（在HLAPRED中发现的）已发表的可导致自身免疫性疾病的肽段后，Th表位必须覆盖上述CTL混合表位。使用多种方法预测了二级结构、疏水区、柔韧性、表面概率和B细胞表位。从1740多个肽段中选择了10个T细胞表位的混合肽段。它们位于抗GP IIb/IIIa - 人源的aa9 - 115、aa24 - 38、aa50 - 64、aa65 - 81、aa109 - 121以及抗GP IIb/IIIa - 小鼠源的aal - 15、aa26 - 40、aa46 - 60、aa68 - 82、aa93 - 107处。抗GP IIb/IIIa - 人源的B细胞表位可能位于aa5 - 9、aa22 - 30、aa40 - 46、aa55 - 71、aa80 - 90、aa100 - 105、aa110 - 115；抗GP IIb/IIIa - 人源的表位可能位于aa5 - 10、aa38 - 43、aa58 - 70、aa77 - 84以及aa99 - 105处。
