Jafarpour Sima, Ayat Hoda, Ahadi Ali Mohammad
Department of Genetics, Faculty of Science, Shahrekord University , Shahrekord, Iran .
Viral Immunol. 2015 Jul-Aug;28(6):325-30. doi: 10.1089/vim.2014.0152. Epub 2015 May 12.
Rotavirus is the major etiologic factor of severe diarrheal disease. Natural infection provides protection against subsequent rotavirus infection and diarrhea. This research presents a new vaccine designed based on computational models. In this study, three types of epitopes are considered-linear, conformational, and combinational-in a proposed model protein. Several studies on rotavirus vaccines have shown that VP6 and VP4 proteins are good candidates for vaccine production. In the present study, a fusion protein was designed as a new generation of rotavirus vaccines by bioinformatics analyses. This model-based study using ABCpred, BCPREDS, Bcepred, and Ellipro web servers showed that the peptide presented in this article has the necessary properties to act as a vaccine. Prediction of linear B-cell epitopes of peptides is helpful to investigate whether these peptides are able to activate humoral immunity.
轮状病毒是严重腹泻疾病的主要病因。自然感染可提供针对后续轮状病毒感染和腹泻的保护。本研究提出了一种基于计算模型设计的新型疫苗。在本研究中，在一个提议的模型蛋白中考虑了三种类型的表位——线性、构象和组合表位。多项关于轮状病毒疫苗的研究表明，VP6和VP4蛋白是疫苗生产的良好候选物。在本研究中，通过生物信息学分析设计了一种融合蛋白作为新一代轮状病毒疫苗。使用ABCpred、BCPREDS、Bcepred和Ellipro网络服务器进行的基于模型的研究表明，本文中呈现的肽具有作为疫苗的必要特性。预测肽的线性B细胞表位有助于研究这些肽是否能够激活体液免疫。
