Wang Cui, Li Huan, Wang Qinqi, Wei Ping
College of Biotechnology and Pharmaceutical Engneering, Nanjing University of Technology, Nanjing 210009, China.
Sheng Wu Gong Cheng Xue Bao. 2010 Aug;26(8):1074-9.
Using 50% biogas slurry as basic medium, we investigated the effect of pH on the growth and lipid accumulation of Chlorella vulgaris. Setting two-group experiments, one was only control the initial medium pH, the initial pH was set at 6.0, 6.5, 7.0, 7.5, 8.0, and 8.5, respectively. One was control the medium pH constant, set constant pH at 6.0, 6.5, 7.0, 7.5, 8.0, and 8.5, respectively. Using HCl and NaOH regulated the pH. Results showed that algae Chlorella vulgaris grows better at pH 6.5 and 7.0, accumulate the lipid at pH 7.0-8.5, so the optimal pH for the growth and the lipid accumulation of Chlorella vulgaris was 7.0. The average removal rate of nitrate from biogas slurry was 95%, phosphate was 97%.
以50%的沼液为基础培养基，研究了pH对小球藻生长和脂质积累的影响。设置两组实验，一组仅控制初始培养基pH，初始pH分别设置为6.0、6.5、7.0、7.5、8.0和8.5。另一组控制培养基pH恒定，分别将恒定pH设置为6.0、6.5、7.0、7.5、8.0和8.5。用HCl和NaOH调节pH。结果表明，小球藻在pH 6.5和7.0时生长较好，在pH 7.0 - 8.5时积累脂质，因此小球藻生长和脂质积累的最佳pH为7.0。沼液中硝酸盐的平均去除率为95%，磷酸盐为97%。
