Zamree Nur Diyanah, Puasa Nurul Aini, Lim Zheng Syuen, Wong Chiew-Yen, Shaharuddin Noor Azmi, Zakaria Nur Nadhirah, Merican Faradina, Convey Peter, Ahmad Syahida, Shaari Hasrizal, Azmi Alyza Azzura, Ahmad Siti Aqlima, Zulkharnain Azham
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.
School of Health Sciences, International Medical University, Bukit Jalil 57000, Kuala Lumpur, Malaysia.
Plants (Basel). 2023 Jul 3;12(13):2536. doi: 10.3390/plants12132536.
Research has confirmed that the utilisation of Antarctic microorganisms, such as bacteria, yeasts and fungi, in the bioremediation of diesel may provide practical alternative approaches. However, to date there has been very little attention towards Antarctic microalgae as potential hydrocarbon degraders. Therefore, this study focused on the utilisation of an Antarctic microalga in the bioremediation of diesel. The studied microalgal strain was originally obtained from a freshwater ecosystem in Paradise Bay, western Antarctic Peninsula. When analysed in systems with and without aeration, this microalgal strain achieved a higher growth rate under aeration. To maintain the growth of this microalga optimally, a conventional one-factor-at a-time (OFAT) analysis was also conducted. Based on the optimized parameters, algal growth and diesel degradation performance was highest at pH 7.5 with 0.5 mg/L NaCl concentration and 0.5 g/L of NaNO as a nitrogen source. This currently unidentified microalga flourished in the presence of diesel, with maximum algal cell numbers on day 7 of incubation in the presence of 1% / diesel. Chlorophyll , and carotenoid contents of the culture were greatest on day 9 of incubation. The diesel degradation achieved was 64.5% of the original concentration after 9 days. Gas chromatography analysis showed the complete mineralisation of C-C hydrocarbon chains. Fourier transform infrared spectroscopy analysis confirmed that strain WCY_AQ5_3 fully degraded the hydrocarbon with bioabsorption of the products. Morphological and molecular analyses suggested that this spherical, single-celled green microalga was a member of the genus . The data obtained confirm that this microalga is a suitable candidate for further research into the degradation of diesel in Antarctica.
研究证实，利用南极微生物，如细菌、酵母和真菌，进行柴油生物修复可能提供切实可行的替代方法。然而，迄今为止，作为潜在的碳氢化合物降解者，南极微藻很少受到关注。因此，本研究聚焦于利用一种南极微藻进行柴油生物修复。所研究的微藻菌株最初取自南极半岛西部天堂湾的一个淡水生态系统。在有曝气和无曝气的系统中进行分析时，该微藻菌株在曝气条件下实现了更高的生长速率。为了最优地维持这种微藻的生长，还进行了传统的单因素逐一分析。基于优化后的参数，在pH 7.5、NaCl浓度为0.5 mg/L以及以0.5 g/L硝酸钠作为氮源的条件下，藻类生长和柴油降解性能最佳。这种目前身份不明的微藻在柴油存在的情况下生长旺盛，在含有1%柴油的培养物中培养7天时藻类细胞数量最多。培养物的叶绿素a、叶绿素b和类胡萝卜素含量在培养第9天时最高。9天后实现的柴油降解量为原始浓度的64.5%。气相色谱分析表明C-C碳氢化合物链完全矿化。傅里叶变换红外光谱分析证实，菌株WCY_AQ5_3通过对产物的生物吸收完全降解了碳氢化合物。形态学和分子分析表明，这种球形的单细胞绿色微藻是小球藻属的一员。所获得的数据证实，这种微藻是进一步研究南极柴油降解的合适候选对象。
