Ljunghusen O, Johansson A, Skogh T
Department of Medical Microbiology, Faculty of Health Sciences, University of Linköping, Sweden.
J Immunol Methods. 1990 Mar 27;128(1):1-7. doi: 10.1016/0022-1759(90)90457-7.
Fluorescein isothiocyanate (FITC)-labelled model antigens (Ags) were used to form soluble IgG immune complexes (ICs) in vitro. The Ag and IC preparations were found to be excellent alternatives to isotope-tagged preparations for the study of plasma clearance after intravenous injection in rats. Ag and IC were eliminated from the blood at the same rate. By direct fluorescence microscopy the FITC-labelled IgG ICs could easily be detected in hepatic non-parenchymal liver cells 30 min after intravenous injection, whereas uncomplexed Ag was apparently taken up by the liver to a much lesser extent.
异硫氰酸荧光素（FITC）标记的模型抗原（Ag）用于在体外形成可溶性IgG免疫复合物（IC）。在研究大鼠静脉注射后的血浆清除率时，发现Ag和IC制剂是同位素标记制剂的极佳替代品。Ag和IC以相同的速率从血液中清除。通过直接荧光显微镜观察，静脉注射30分钟后，可在肝非实质细胞中轻松检测到FITC标记的IgG IC，而未结合的Ag被肝脏摄取的程度明显要小得多。
