Steven W M
Acta Anat (Basel). 1986;125(3):201-7. doi: 10.1159/000146162.
Although sheep erythrocytes (SRBCs) are extensively used as an antigen in immunological studies, their histological distribution in lymphoid tissues has received little attention. The objective of this study was to determine the histological distribution of injected SRBCs in rat spleen. SRBCs were labelled with fluorescein isothiocyanate (FITC) to facilitate their identification in spleen sections with fluorescence microscopy. Rats received intravenous injections of FITC-labelled SRBCs and were sacrificed at various periods after injection. At 15 min, SRBCs were distributed throughout the marginal zone and red pulp. After 4 h, intact SRBCs were located mainly in the red pulp, while the marginal zone contained fluorescent flocculent material. At later periods this material was present in the periarterial lymphatic sheath (PALS) and in the light and dark zones of the germinal centers. By 12 h, the most intensely labelled areas in the white pulp were the crescent-shaped light zones. In 12 and 24 h, the PALS contained numerous foci of labelled granules. Some of the dark zones also contained label. After 48 h, the only areas containing label were the light zones of the germinal centers.
尽管绵羊红细胞（SRBCs）在免疫学研究中被广泛用作抗原，但其在淋巴组织中的组织学分布却很少受到关注。本研究的目的是确定注射的SRBCs在大鼠脾脏中的组织学分布。用异硫氰酸荧光素（FITC）标记SRBCs，以便通过荧光显微镜在脾脏切片中对其进行识别。给大鼠静脉注射FITC标记的SRBCs，并在注射后的不同时间段处死。15分钟时，SRBCs分布于整个边缘区和红髓。4小时后，完整的SRBCs主要位于红髓，而边缘区含有荧光絮状物质。在随后的时间段，这种物质出现在动脉周围淋巴鞘（PALS）以及生发中心的明区和暗区。到12小时时，白髓中标记最强烈的区域是新月形的明区。在12小时和24小时时，PALS含有许多标记颗粒的病灶。一些暗区也含有标记。48小时后，唯一含有标记的区域是生发中心的明区。
