Goldblatt D, Seymour N D, Levinsky R J, Turner M W
Department of Immunology, University of London, U.K.
J Immunol Methods. 1990 Apr 17;128(2):219-25. doi: 10.1016/0022-1759(90)90213-f.
An ELISA procedure to determine the distribution of human IgG subclass antibodies directed against the gram-negative bacterium Branhamella catarrhalis has been developed using commercially available monoclonal anti-IgG subclass antibodies. Using whole bacteria as coating antigen the specificity of the assay was determined and showed minimal cross-reactivity with a range of other bacteria. Estimations of IgG1, IgG2, IgG3, IgG4 and total IgG antibodies directed against this antigen were performed. All normal adult sera tested had measurable antibody levels of specific IgG1, IgG2, IgG3 and total IgG. Specific IgG4 was undetectable in the majority of adult sera. These assays will be of value for investigation of both children and adults with suspected immunodeficiency and recurrent upper respiratory tract infection.
已利用市售抗IgG亚类单克隆抗体开发出一种酶联免疫吸附测定（ELISA）程序，用于确定针对革兰氏阴性菌卡他莫拉菌的人IgG亚类抗体的分布情况。使用全菌作为包被抗原，确定了该检测方法的特异性，结果显示其与一系列其他细菌的交叉反应性极小。对针对该抗原的IgG1、IgG2、IgG3、IgG4和总IgG抗体进行了测定。所有检测的正常成人血清中，特异性IgG1、IgG2、IgG3和总IgG的抗体水平均可测量。大多数成人血清中未检测到特异性IgG4。这些检测方法对于调查疑似免疫缺陷和复发性上呼吸道感染的儿童和成人具有重要价值。
