Shagimardanova E I, Gusev O A, Sychev V N, Levinskikh M A, Sharipova M R, Il'inskaia O N, Bingham G, Sugimoto M
Mol Biol (Mosk). 2010 Sep-Oct;44(5):831-8.
Transcriptome of barley Hordeum vulgare grown aboard International Space Station (ISS) was analyzed by means of microarray. It was revealed 500 genes with mRNA level, changed more than two folds in space environment. Among them are genes encoding stress response proteins, videlicet Heat Shock Proteins (HSP), Pathogenesis-Related Proteins (PR) and Antioxidant Proteins. Further analysis of these genes by real time PCR showed enhanced transcription level of Reactive oxygen Species (ROS) scavenging genes. The mRNA level of superoxide dismutase (sod) was 6 folds higher in space environment when compare to Earth conditions. Glutamyl transferase gene expression was enhanced 24 times in space. Transcription of catalase gene (cat) was increased 18 times and of ascorbate peroxidase was increased 3 times in space in comparison with ground control. For the first time it was shown that space flight environment may induce oxidative stress in plants.
利用微阵列分析了在国际空间站（ISS）上生长的大麦（Hordeum vulgare）转录组。结果发现有500个基因的mRNA水平在太空环境中变化超过两倍。其中包括编码应激反应蛋白的基因，即热休克蛋白（HSP）、病程相关蛋白（PR）和抗氧化蛋白。通过实时PCR对这些基因的进一步分析表明，活性氧（ROS）清除基因的转录水平增强。与地球条件相比，超氧化物歧化酶（sod）的mRNA水平在太空环境中高出6倍。谷氨酰胺转移酶基因表达在太空中增强了24倍。与地面对照相比，过氧化氢酶基因（cat）的转录在太空中增加了18倍，抗坏血酸过氧化物酶增加了3倍。首次表明太空飞行环境可能诱导植物产生氧化应激。
