Talalaiev Oleksandr, Korduym Elizabeth
Department of Cell Biology and Anatomy; M. G. Kholodny Institute of Botany of NAS of Ukraine; Kyiv, Ukraine.
Plant Signal Behav. 2014 Apr 30;9. doi: 10.4161/psb.29035.
Plant cells respond to stress conditions, such as high temperatures, by synthesizing small heat shock proteins (sHSPs). sHSPs are molecular chaperones that assist in protein folding and prevent irreversible protein aggregation. Although many sHSP genes are temperature-inducible, other variables, such as altered gravity, can induce significant changes in plant cell gene expression. Furthermore, not all subfamilies of sHSP genes share the same expression pattern. The objective of our research was to determine the effect of simulated microgravity (clinorotation) on the expression of sHSP gene subfamilies with different subcellular locations in etiolated pea (Pisum sativum) seedlings. sHSP gene expression levels were examined using quantitative real-time RT-PCR (qPCR). qPCR results demonstrated that sHSP genes were constitutively expressed in seedlings. High temperatures increased the expression of sHSP genes by several thousand-fold. However, simulated microgravity did not have any significant effects on sHSP gene expression.
植物细胞通过合成小分子热激蛋白（sHSPs）来应对高温等胁迫条件。sHSPs是分子伴侣，有助于蛋白质折叠并防止蛋白质发生不可逆聚集。尽管许多sHSP基因是温度诱导型的，但其他变量，如重力改变，也能诱导植物细胞基因表达发生显著变化。此外，并非所有sHSP基因亚家族都具有相同的表达模式。我们研究的目的是确定模拟微重力（回转器旋转）对黄化豌豆（Pisum sativum）幼苗中不同亚细胞定位的sHSP基因亚家族表达的影响。使用定量实时逆转录PCR（qPCR）检测sHSP基因的表达水平。qPCR结果表明，sHSP基因在幼苗中组成性表达。高温使sHSP基因的表达增加了数千倍。然而，模拟微重力对sHSP基因表达没有任何显著影响。
