Wang Qian, Xie Mingjie
College of Life Science, Liaoning Normal University, Dalian 116029, China.
Wei Sheng Wu Xue Bao. 2010 Sep;50(9):1180-4.
To investigate the antibacterial activity and mechanism of luteolin on Staphylococcus aureus.
The antibacterial activity and mechanism experiments were carried out by using 2, 3, 5-triphenyl tetrazolium chloride (TTC), determining membrane penetrability, the change of SDS-PAGE protein spectra and 4 6-diamidino-2-phenylindole (DAPI) staining assay.
Luteolin could affect the membrane permeability of Staphylococcus aureus, but not destroy the membrane integrity directly. After treated with luteolin for 16 hours, the total content of proteins decreased to 64.54%, the quantity of both DNA and RNA reduced to 48.44% and 39.35% respectively. The activity of DNA topoisomerase I and II was inhibited completely by 1.6 mg/mL luteolin.
Luteolin showed obvious antibacterial activity against Staphylococcus aureus. The antibacterial mechanism of luteolin is that it could inhibit the activity of DNA topoisomerase I and II, which resulted in some decrease in the nucleic acid and protein synthesis.
研究木犀草素对金黄色葡萄球菌的抗菌活性及作用机制。
采用氯化三苯基四氮唑（TTC）法、测定膜通透性、十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）蛋白质谱变化及4,6 - 二脒基 - 2 - 苯基吲哚（DAPI）染色法进行抗菌活性及作用机制实验。
木犀草素可影响金黄色葡萄球菌的膜通透性，但不直接破坏膜完整性。用木犀草素处理16小时后，蛋白质总含量降至64.54%，DNA和RNA含量分别降至48.44%和39.35%。1.6 mg/mL木犀草素可完全抑制DNA拓扑异构酶I和II的活性。
木犀草素对金黄色葡萄球菌具有明显的抗菌活性。木犀草素的抗菌机制是抑制DNA拓扑异构酶I和II的活性，导致核酸和蛋白质合成有所减少。
