Wu Hua-Zhang, Fei Hong-Jun, Zhao Yun-li, Liu Xiao-jia, Huang Yin-jiu, Wu Shou-wei
Department of Bioscience, Bengbu Medical College, Bengbu 233030, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2012 May;43(3):386-90.
To investigate the antibacterial mechanism of sulforaphaneon (SFN) on Escherichia coli.
To determine membrane penetrability, changes of SDS-PAGE protein spectra, soluble protein and alkaline phosphatase and reducing sugar were determined. Cellular nucleic acid synthesis was detected by 4, 6- diamidino-2-phenylindole (DAPI) staining assay.
SFN affected the membrane permeability of Escherichia coli. Ions and small molecules could leak out of the cells. But it did not destroy the membrane integrity directly. After 16 hours of treatment with SFN, the total contents of intracellular and extracellular proteins decreased by 42.5% and 17.6%, respectively, while the quantity of DNA and RNA reduced by 34.8% and 48.5% respectively.
SFN can affect cell membrane permeability, material and energy metabolism and inhibit the synthesis of nucleic acid and protein.
研究萝卜硫素（SFN）对大肠杆菌的抗菌机制。
测定膜通透性、SDS-PAGE蛋白质谱变化、可溶性蛋白质、碱性磷酸酶和还原糖。通过4,6-二脒基-2-苯基吲哚（DAPI）染色法检测细胞核酸合成。
SFN影响大肠杆菌的膜通透性。离子和小分子可从细胞中泄漏出来。但它不会直接破坏膜的完整性。用SFN处理16小时后，细胞内和细胞外蛋白质的总含量分别下降了42.5%和17.6%，而DNA和RNA的量分别减少了34.8%和48.5%。
SFN可影响细胞膜通透性、物质和能量代谢，并抑制核酸和蛋白质的合成。
