Li Pengcheng, Ye Xiaolan, Wang Zhisheng, Yu Qinghua, Yang Qiang
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Wei Sheng Wu Xue Bao. 2010 Sep;50(9):1226-31.
S-layer proteins of Lactobacillus acidophilus were extracted and purified, then the effect of Lactobacillus acidophilus and its S-layer proteins against the adhesion and invasion of salmonella typhimurium to caco-2 cells were investigated.
S-layer proteins were purified by anion-exchange column {diethylaminoethyl (DEAE) DE52}, and the inhibition of Lactobacillus acidophilus and its S-layer proteins were studied against the adhesion and invasion of salmonella typhimurium on Caco-2 cells.
S-layer proteins exhibited strongly inhibitory effects of adhesive and invading properties of Salmonella typhimurium. In the adhesive experiments (competitive, exclusive and displacement), Salmonella typhimurium adhesion was reduced by S-layer proteins and the ability of adherence to Caco-2 cells were 1.17% +/- 5.97%, 8.71% +/- 1.36% and 10.56% +/- 0.92%, respectively (P < 0.01). The influence to inhibit the competitive adhesion of Salmonella typhimurium was optimal. Furthermore, the S-layer proteins showed a stronger effect than Lactobacillus acidophilus to inhibit Salmonella typhimurium adhesion on Caco-2 monolayers (P < 0.01). Moreover, invasion of Salmonella typhimurium to Caco-2 monolayers was inhibited by S-layer proteins.
S-layer proteins inhibited adherence and invasion of Salmonella typhimurium. The result can merit a highlight for preventive or probiotic therapy in human or animals with disease caused by Salmonella typhimurium.
提取并纯化嗜酸乳杆菌的S层蛋白，研究嗜酸乳杆菌及其S层蛋白对鼠伤寒沙门氏菌黏附及侵袭Caco-2细胞的影响。
采用阴离子交换柱（二乙氨基乙基（DEAE）DE52）纯化S层蛋白，研究嗜酸乳杆菌及其S层蛋白对鼠伤寒沙门氏菌黏附及侵袭Caco-2细胞的抑制作用。
S层蛋白对鼠伤寒沙门氏菌的黏附及侵袭特性具有强烈的抑制作用。在黏附实验（竞争、排他和置换）中，S层蛋白可降低鼠伤寒沙门氏菌的黏附，其黏附于Caco-2细胞的能力分别为1.17%±5.97%、8.71%±1.36%和10.56%±0.92%（P<0.01）。对抑制鼠伤寒沙门氏菌竞争黏附的影响最佳。此外，S层蛋白在抑制鼠伤寒沙门氏菌黏附Caco-2单层细胞方面比嗜酸乳杆菌表现出更强的作用（P<0.01）。而且，S层蛋白可抑制鼠伤寒沙门氏菌对Caco-2单层细胞的侵袭。
S层蛋白可抑制鼠伤寒沙门氏菌的黏附和侵袭。该结果对于由鼠伤寒沙门氏菌引起疾病的人类或动物的预防或益生菌治疗具有重要意义。
