Microbiology & Fermentation Laboratory, Calpis Co, Ltd, 11–10, 5-Chome, Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252–0206, Japan.
J Biosci Bioeng. 2011 Oct;112(4):333-7. doi: 10.1016/j.jbiosc.2011.06.001. Epub 2011 Jul 16.
The adhesive activities of eight Lactobacillus acidophilus strains toward intestinal epithelial Caco-2 cells were studied to understand the probiotic characteristics of the L. acidophilus L-92 strain. Most of the strains, including L-92, showed high adhesive activity; CP23 showed the lowest adhesive activity. CP23 was selected for comparative analysis of cell wall-associated proteins versus the L-92 strain. Cell wall-associated proteins extracted from L-92 and CP23 were subjected to two-dimensional electrophoresis, and major spots observed in the former were compared to the corresponding spots in the latter. To understand the effects of key components of L-92 on its adhesion to Caco-2 cells, 18 spots with stronger signals in L-92 than those in CP23 were identified by a MALDI-TOF/TOF of Ultraflex analysis. Among the identified proteins of L-92, surface-layer protein A (SlpA) was considered strongly involved in adhesion in the eight L. acidophilus strains. To study the importance of SlpA in the adhesion of L. acidophilus, the amounts of SlpA proteins in LiCl extracts of the eight strains were compared by SDSpolyacrylamide gel electrophoresis. As a result, the adhesive abilities of L. acidophilus strains to Caco-2 cells correlated closely to the amount of SlpA in the cells and the productivity of IL-12, an inflammatory cytokine, in all eight strains. These results strongly suggested that SlpA in L. acidophilus might play a key role in its attachment to Caco-2 cells and in the release of IL-12 from dendritic cells.
研究了 8 株嗜酸乳杆菌（Lactobacillus acidophilus）对肠道上皮细胞 Caco-2 的黏附活性，以了解嗜酸乳杆菌 L-92 株的益生菌特性。包括 L-92 在内的大多数菌株均表现出较高的黏附活性；CP23 的黏附活性最低。选择 CP23 进行与 L-92 菌株的细胞壁相关蛋白的比较分析。从 L-92 和 CP23 中提取细胞壁相关蛋白，进行二维电泳，比较前者中的主要斑点与后者中的相应斑点。为了了解 L-92 关键成分对其与 Caco-2 细胞黏附的影响，通过 MALDI-TOF/TOF Ultraflex 分析鉴定了 L-92 中比 CP23 信号更强的 18 个斑点。在鉴定出的 L-92 蛋白中，认为表面层蛋白 A（SlpA）强烈参与了 8 株嗜酸乳杆菌的黏附。为了研究 SlpA 在嗜酸乳杆菌黏附中的重要性，比较了 8 株菌 LiCl 提取物中 SlpA 蛋白的含量。结果表明，嗜酸乳杆菌菌株对 Caco-2 细胞的黏附能力与细胞中 SlpA 的含量以及 8 株菌中炎症细胞因子 IL-12 的产生密切相关。这些结果强烈表明，嗜酸乳杆菌中的 SlpA 可能在其与 Caco-2 细胞的黏附和树突状细胞中 IL-12 的释放中发挥关键作用。
