Gutkowska J, Boucher R, Demassieux S, Garcia R, Genest J
Can J Biochem. 1978 Aug;56(8):769-73. doi: 10.1139/o78-116.
A sensitive radioimmunoassay for the measurement of tonin is described. It is based on the use of antibodies produced in rabbits against highly purified tonin obtained from rat submaxillary gland. A radioiodinated enzyme with high specific activity was obtained by the chloramine-T method. Optimal conditions for radioimmunoprecipitation were established and the double-antibody method was used to separate bound and free tonin. The radioimmunoassay may be used to measure tonin in amounts as low as 150 pg. This radioimmunoassay was applied to the measurement of tonin in rat saliva and in homogenates of submaxillary glands. Excellent correlation was found between tonin activity measured fluorometrically and tonin concentration obtained by the radioimmunoassay.
本文描述了一种用于测定托宁的灵敏放射免疫分析方法。该方法基于使用兔产生的针对从大鼠颌下腺获得的高度纯化托宁的抗体。通过氯胺-T法获得了具有高比活性的放射性碘化酶。确定了放射免疫沉淀的最佳条件，并采用双抗体法分离结合态和游离态的托宁。该放射免疫分析方法可用于测定低至150皮克的托宁。此放射免疫分析方法应用于大鼠唾液和颌下腺匀浆中托宁的测定。通过荧光法测定的托宁活性与通过放射免疫分析获得的托宁浓度之间发现了良好的相关性。
