Ono N, Ko K K, Hosaka Y
Department of Preventive Medicine, Osaka University, Japan.
J Virol. 1990 May;64(5):2442-7. doi: 10.1128/JVI.64.5.2442-2447.1990.
Lysis of virus-infected L929 target cells transfected with the H-2 class II IAk gene by class II-restricted influenza virus-specific murine cytotoxic T lymphocyte (CTL) clones was studied by electron microscopy and compared with lysis of L929 cells by class I-restricted CTL clones. T lymphocytes predominantly approached the basal surface of target cells grown on a plastic dish and also approached uninfected L929 target cells, although virus maturation exhibited no polarity with respect to the cell surface site. After incubation for 30 min, the target cell nuclei began to change: chromatin became irregularly redistributed and aggregated, and the nuclei appeared swollen. Later, electron-dense and -light areas of nuclei became segregated, and the cytoplasm became disorganized with many vacuoles. The ultrastructural changes of target cells during lysis by class I- and class II-restricted CTL clones appeared to be similar. These findings and other cytotoxicity data of class I and class II CTLs are discussed.
通过电子显微镜研究了由II类限制性流感病毒特异性鼠细胞毒性T淋巴细胞（CTL）克隆对转染了H-2 II类IAk基因的病毒感染L929靶细胞的裂解作用，并将其与I类限制性CTL克隆对L929细胞的裂解作用进行了比较。T淋巴细胞主要接近生长在塑料培养皿上的靶细胞的基底表面，并且也接近未感染的L929靶细胞，尽管病毒成熟在细胞表面位点方面没有表现出极性。孵育30分钟后，靶细胞核开始变化：染色质变得不规则地重新分布并聚集，细胞核出现肿胀。后来，细胞核的电子致密区和明亮区变得分离，细胞质变得紊乱并出现许多空泡。I类和II类限制性CTL克隆裂解靶细胞期间的超微结构变化似乎相似。讨论了这些发现以及I类和II类CTL的其他细胞毒性数据。
