Petrovic A, Abramovic M, Mihailovic D, Gligorijevic J, Zivkovic V, Mojsilovic M, Ilic I
Institute of Histology and Embryology, Faculty of Medicine, University of Nis, 18000 Nis, Serbia.
Biotech Histochem. 2011 Dec;86(6):429-35. doi: 10.3109/10520295.2010.528026. Epub 2010 Nov 22.
The application of "histochemical" staining procedures has been substantially replaced by immunostaining of specific molecular tissue components. The limited range of colors resulting from routine immunohistochemistry, however, can limit assessment of the general microscopic tissue organization. Consequently we have adapted a polychromatic histochemical counterstaining procedure based on Movat's pentachrome staining sequence for use with immunohistochemical procedures. The value of Movat's original method when applied as an immunohistochemical counterstain is limited by its use of iron hematoxylin and by fact that the resulting color combination is difficult to distinguish from the colors of routine immunohistochemical staining. Our variant pentachrome stains the same tissue components as Movat's stain; however, owing to a modification of the acid fuchsin staining step, it provides a strong color contrast with the reaction product resulting from immunostaining using diaminobenzidine as the chromogen. Multicolor counterstaining for immunohistochemistry offers a new approach to tissue analysis, especially when stromal-epithelial relations of normal and neoplastic tissues are considered.
“组织化学”染色程序的应用已基本被特定分子组织成分的免疫染色所取代。然而，常规免疫组织化学产生的颜色范围有限，可能会限制对一般微观组织结构的评估。因此，我们采用了一种基于莫瓦特氏五色染色序列的多色组织化学复染程序，用于免疫组织化学程序。莫瓦特氏原方法作为免疫组织化学复染剂使用时，其价值受到苏木精铁的使用以及所得颜色组合难以与常规免疫组织化学染色颜色区分开来的限制。我们的改良五色染色法与莫瓦特氏染色法染色相同的组织成分；然而，由于酸性品红染色步骤的改进，它与使用二氨基联苯胺作为显色剂的免疫染色反应产物形成了强烈的颜色对比。免疫组织化学的多色复染为组织分析提供了一种新方法，尤其是在考虑正常组织和肿瘤组织的基质 - 上皮关系时。
