Petticrew Research Laboratory, Department of Biochemistry and Molecular Biology, Boonshoft School of Medicine Wright State University, Dayton, OH, USA.
Photochem Photobiol. 2011 Jan-Feb;87(1):151-9. doi: 10.1111/j.1751-1097.2010.00844.x. Epub 2010 Nov 23.
Crystallins in the retina may serve a chaperone-like protective function. In this study we measured mRNA levels for alpha-, beta- and gamma-crystallins in rat retinas following treatment with potentially damaging levels of light. We also determined crystallin protein patterns in photoreceptor cell rod outer segments (ROSs) isolated from rats exposed to intense light. Weanling albino rats were maintained in a dim cyclic light environment or in darkness for 40days. At P60 animals were treated with intense visible light, for as long as 8h, beginning at various times of the day or night. Retinas were excised immediately after light treatment and used for quantitative RT-PCR, or to prepare ROSs for western analysis. Some eyes were frozen in OCT for crystallin immunohistochemistry. Intense light exposure led to increases in mRNA expression for all retinal crystallins and to changes in ROS crystallin immunoreactivity. These light-induced changes were found to depend on the time of day that exposure started, duration of light treatment and previous light rearing history. We suggest that crystallin synthesis in retina exhibits a dependence on both light stress and circadian rhythm and that within photoreceptor cells crystallins appear to migrate in a light-independent, circadian fashion.
视网膜中的晶体蛋白可能具有伴侣样保护功能。在这项研究中，我们测量了在给予潜在有害光剂量后，大鼠视网膜中α、β和γ晶体蛋白的 mRNA 水平。我们还测定了来自暴露于强光的大鼠光感受器细胞视杆外节（ROS）的晶体蛋白模式。我们将白化幼鼠维持在昏暗的循环光环境或黑暗中 40 天。在 P60 时，动物接受强烈的可见光照射，时间长达 8 小时，开始于白天或晚上的不同时间。在光处理后立即切除视网膜，用于定量 RT-PCR，或用于制备 ROS 进行 Western 分析。一些眼睛用 OCT 冷冻用于晶体蛋白免疫组织化学。强烈的光照暴露导致所有视网膜晶体蛋白的 mRNA 表达增加，并导致 ROS 晶体蛋白免疫反应性发生变化。这些光诱导的变化被发现取决于暴露开始的时间、光处理的持续时间和以前的光饲养史。我们认为，视网膜中的晶体蛋白合成表现出对光应激和昼夜节律的依赖性，并且在光感受器细胞中，晶体蛋白似乎以非光依赖的、昼夜节律的方式迁移。
