Sakaguchi Hirokazu, Miyagi Masaru, Darrow Ruth M, Crabb John S, Hollyfield Joe G, Organisciak Daniel T, Crabb John W
Exp Eye Res. 2003 Jan;76(1):131-3. doi: 10.1016/s0014-4835(02)00249-x.
Toward a better understanding of light-induced photoreceptor damage, the crystallin content of rat retina was examined following intense light exposure. Nine crystallin species were identified by mass spectrometric analysis of rat retina fractionated by 2D gel electrophoresis. The Coomassie blue staining intensity of all crystallin 2D gel components was 2- to 3-fold greater in light exposed than in control retinas. Following light exposure, anti-alphaB-crystallin immunoreactivity was increased in rod outer segments and retinal pigment epithelium. These findings support a possible role for crystallins in protecting photoreceptors from light damage.
为了更好地理解光诱导的光感受器损伤，在强光照射后检测了大鼠视网膜的晶状体蛋白含量。通过二维凝胶电泳分离大鼠视网膜，经质谱分析鉴定出九种晶状体蛋白。与对照视网膜相比，所有晶状体蛋白二维凝胶组分的考马斯亮蓝染色强度在光照后增加了2至3倍。光照后，视杆细胞外段和视网膜色素上皮中的抗αB晶状体蛋白免疫反应性增强。这些发现支持晶状体蛋白在保护光感受器免受光损伤方面可能发挥的作用。
