Vanĕcek J, Vollrath L
Department of Anatomy, Johannes Gutenberg University, Mainz, F.R.G.
Neurosci Lett. 1990 Mar 2;110(1-2):199-203. doi: 10.1016/0304-3940(90)90811-m.
In pituitary glands of immature rats prelabeled in vitro with [3H]arachidonic acid, melatonin diminished the luteinizing hormone-releasing hormone (LHRH)-induced increase in [3H]diacylglycerol accumulation as well as [3H]arachidonic acid release from the tissue. Melatonin reduced also LHRH-stimulated incorporation of [3H]glycerol into pituitary [3H]diacylglycerol. The effect was day-time dependent: in the evening experiment melatonin was effective at 0.1 nM concentration while in the morning it had no effect even at 10 nM concentration. The effect of melatonin was also abolished by pretreatment with pertussis toxin. Diacylglycerol and/or arachidonic acid might serve as 2nd messengers transducing the effect of melatonin at the cellular level.
在体外预先用[3H]花生四烯酸标记的未成熟大鼠垂体中，褪黑素减少了促黄体生成素释放激素（LHRH）诱导的[3H]二酰甘油积累增加以及组织中[3H]花生四烯酸的释放。褪黑素还降低了LHRH刺激的[3H]甘油掺入垂体[3H]二酰甘油的量。该效应具有日间依赖性：在傍晚实验中，褪黑素在0.1 nM浓度时有效，而在早晨即使在10 nM浓度时也无作用。用百日咳毒素预处理也消除了褪黑素的作用。二酰甘油和/或花生四烯酸可能作为第二信使在细胞水平转导褪黑素的作用。
