反密码子的改变将大肠杆菌甲硫氨酸起始tRNA（tRNA^（mMet））的识别对象从甲硫氨酸转换为苏氨酸。
An anticodon change switches the identity of E. coli tRNA(mMet) from methionine to threonine.
作者信息
Schulman L H, Pelka H
机构信息
Department of Developmental Biology and Cancer, Albert Einstein College of Medicine, Bronx, NY 10461.
出版信息
Nucleic Acids Res. 1990 Jan 25;18(2):285-9. doi: 10.1093/nar/18.2.285.
Abstract
Recent evidence indicates that the anticodon may often play a crucial role in selection of tRNAs by aminoacyl-tRNA synthetases. In order to quantitate the contribution of the anticodon to discrimination between cognate and noncognate tRNAs by E. coli threonyl-tRNA synthetase, derivatives of the E. coli elongator methionine tRNA (tRNA(mMet)) containing wild type and threonine anticodons have been synthesized in vitro and assayed for threonine acceptor activity. Substitution of the threonine anticodon GGU for the methionine anticodon CAU increased the threonine acceptor activity of tRNA(mMet) by four orders of magnitude while reducing methionine acceptor activity by an even greater amount. These results indicate that the anticodon is the major element which determines the identity of both threonine and methionine tRNAs.
摘要
最近的证据表明，反密码子在氨酰-tRNA合成酶选择tRNA的过程中可能常常发挥关键作用。为了定量分析反密码子对大肠杆菌苏氨酰-tRNA合成酶区分同源和非同源tRNA的贡献，已在体外合成了含有野生型和苏氨酸反密码子的大肠杆菌延伸甲硫氨酸tRNA（tRNA(mMet)）衍生物，并对其苏氨酸接受活性进行了测定。用苏氨酸反密码子GGU替换甲硫氨酸反密码子CAU，使tRNA(mMet)的苏氨酸接受活性提高了四个数量级，同时使甲硫氨酸接受活性降低得更多。这些结果表明，反密码子是决定苏氨酸和甲硫氨酸tRNA身份的主要因素。
相似文献
1
An anticodon change switches the identity of E. coli tRNA(mMet) from methionine to threonine.反密码子的改变将大肠杆菌甲硫氨酸起始tRNA（tRNA^（mMet））的识别对象从甲硫氨酸转换为苏氨酸。
Nucleic Acids Res. 1990 Jan 25;18(2):285-9. doi: 10.1093/nar/18.2.285.
2
The anticodon contains a major element of the identity of arginine transfer RNAs.反密码子包含精氨酸转运核糖核酸识别的一个主要元件。
Science. 1989 Dec 22;246(4937):1595-7. doi: 10.1126/science.2688091.
3
4
Involvement of the size and sequence of the anticodon loop in tRNA recognition by mammalian and E. coli methionyl-tRNA synthetases.反密码子环的大小和序列在哺乳动物和大肠杆菌甲硫氨酰 - tRNA合成酶识别tRNA中的作用。
Nucleic Acids Res. 1992 Sep 25;20(18):4741-6. doi: 10.1093/nar/20.18.4741.
5
Molecular mimicry in translational control of E. coli threonyl-tRNA synthetase gene. Competitive inhibition in tRNA aminoacylation and operator-repressor recognition switch using tRNA identity rules.大肠杆菌苏氨酰 - tRNA合成酶基因翻译控制中的分子模拟。利用tRNA识别规则在tRNA氨酰化及操纵子 - 阻遏物识别开关中的竞争性抑制作用。
Nucleic Acids Res. 1992 Nov 11;20(21):5633-40. doi: 10.1093/nar/20.21.5633.
6
Identification of the tRNA anticodon recognition site of Escherichia coli methionyl-tRNA synthetase.大肠杆菌甲硫氨酰 - tRNA合成酶tRNA反密码子识别位点的鉴定。
Biochemistry. 1990 Mar 6;29(9):2220-5. doi: 10.1021/bi00461a003.
7
8
Selection of suppressor methionyl-tRNA synthetases: mapping the tRNA anticodon binding site.抑制性甲硫氨酰 - tRNA合成酶的选择：绘制tRNA反密码子结合位点图谱。
Proc Natl Acad Sci U S A. 1991 Jan 1;88(1):291-5. doi: 10.1073/pnas.88.1.291.
9
Initiation of in vivo protein synthesis with non-methionine amino acids.利用非甲硫氨酸氨基酸启动体内蛋白质合成。
Biochemistry. 1990 May 8;29(18):4263-8. doi: 10.1021/bi00470a001.
10
Binding of the anticodon domain of tRNA(fMet) to Escherichia coli methionyl-tRNA synthetase.tRNA（fMet）的反密码子结构域与大肠杆菌甲硫氨酰-tRNA合成酶的结合。
J Mol Biol. 1991 Jul 20;220(2):205-8. doi: 10.1016/0022-2836(91)90003-o.
引用本文的文献
1
Connecting tRNA Charging and Decoding through the Axis of Nucleotide Modifications at Position 37.通过37位核苷酸修饰轴连接tRNA的氨基酸负载与解码过程。
J Mol Biol. 2025 Aug 15;437(16):169095. doi: 10.1016/j.jmb.2025.169095. Epub 2025 Mar 18.
2
Biochemistry of Aminoacyl tRNA Synthetase and tRNAs and Their Engineering for Cell-Free and Synthetic Cell Applications.氨酰-tRNA合成酶与tRNA的生物化学及其在无细胞和合成细胞应用中的工程改造
Front Bioeng Biotechnol. 2022 Jul 1;10:918659. doi: 10.3389/fbioe.2022.918659. eCollection 2022.
3
Whole-Genome Transformation Promotes tRNA Anticodon Suppressor Mutations under Stress.全基因组转化促进应激下 tRNA 反密码子抑制突变。
mBio. 2021 Mar 23;12(2):e03649-20. doi: 10.1128/mBio.03649-20.
4
Strategies for in vitro engineering of the translation machinery.体外翻译机制的工程策略。
Nucleic Acids Res. 2020 Feb 20;48(3):1068-1083. doi: 10.1093/nar/gkz1011.
5
A natural non-Watson-Crick base pair in human mitochondrial tRNAThr causes structural and functional susceptibility to local mutations.人类线粒体 tRNAThr 中的一种自然非 Watson-Crick 碱基对导致其对局部突变的结构和功能易感性。
Nucleic Acids Res. 2018 May 18;46(9):4662-4676. doi: 10.1093/nar/gky243.
6
The methyl group of the N6-methyl-N6-threonylcarbamoyladenosine in tRNA of Escherichia coli modestly improves the efficiency of the tRNA.大肠杆菌转运核糖核酸（tRNA）中N6-甲基-N6-苏氨酰氨甲酰腺苷的甲基基团适度提高了tRNA的效率。
J Bacteriol. 1998 Apr;180(7):1808-13. doi: 10.1128/JB.180.7.1808-1813.1998.
7
Anticodon bases C34 and C35 are major, positive, identity elements in Saccharomyces cerevisiae tRNA(Trp).反密码子的第34位和第35位碱基是酿酒酵母色氨酸转运RNA（tRNA(Trp)）中的主要正向识别元件。
Nucleic Acids Res. 1993 Nov 11;21(22):5079-84. doi: 10.1093/nar/21.22.5079.
8
Seryl-tRNA synthetase from Escherichia coli: implication of its N-terminal domain in aminoacylation activity and specificity.来自大肠杆菌的丝氨酰 - tRNA合成酶：其N端结构域在氨基酰化活性和特异性中的作用
Nucleic Acids Res. 1994 Aug 11;22(15):2963-9. doi: 10.1093/nar/22.15.2963.
9
Identity elements of Saccharomyces cerevisiae tRNA(His).酿酒酵母tRNA（His）的识别元件
Nucleic Acids Res. 1995 Feb 11;23(3):389-94. doi: 10.1093/nar/23.3.389.
10
Porcine E-selectin: cloning and functional characterization.猪E选择素：克隆与功能特性分析
Immunology. 1995 May;85(1):140-5.
本文引用的文献
1
Role of exposed cytosine residues in aminoacylation activity of tRNATrp.暴露的胞嘧啶残基在色氨酸转运RNA氨基酰化活性中的作用。
FEBS Lett. 1981 Sep 28;132(2):349-52. doi: 10.1016/0014-5793(81)81195-7.
2
Specific interaction of anticodon loop residues with yeast phenylalanyl-tRNA synthetase.反密码子环残基与酵母苯丙氨酰 - tRNA合成酶的特异性相互作用。
Biochemistry. 1982 Aug 17;21(17):3921-6. doi: 10.1021/bi00260a003.
3
Cloning and expression of the gene for bacteriophage T7 RNA polymerase.噬菌体T7 RNA聚合酶基因的克隆与表达。
Proc Natl Acad Sci U S A. 1984 Apr;81(7):2035-9. doi: 10.1073/pnas.81.7.2035.
4
Anticodon loop size and sequence requirements for recognition of formylmethionine tRNA by methionyl-tRNA synthetase.甲硫氨酰 - tRNA合成酶识别甲酰甲硫氨酸tRNA的反密码子环大小及序列要求。
Proc Natl Acad Sci U S A. 1983 Nov;80(22):6755-9. doi: 10.1073/pnas.80.22.6755.
5
Normal and mutant glycine transfer RNAs.正常和突变的甘氨酸转运核糖核酸
Nat New Biol. 1971 Oct 27;233(43):274-7. doi: 10.1038/newbio233274a0.
6
Primary structure of a methionine transfer RNA from Escherichia coli.来自大肠杆菌的甲硫氨酸转运核糖核酸的一级结构。
Nature. 1968 Dec 7;220(5171):1039-40. doi: 10.1038/2201039a0.
7
The nucleotide sequence of a threonine transfer ribonucleic acid from Escherichia coli.来自大肠杆菌的苏氨酸转移核糖核酸的核苷酸序列。
J Biol Chem. 1974 Nov 10;249(21):6874-85.
8
Inactivation of valine acceptor ativity by a C-U missense change in the anticodon of yeast valine transfer ribonucleic acid.酵母缬氨酸转移核糖核酸反密码子中的C-U错义变化导致缬氨酸受体活性失活。
J Biol Chem. 1973 Aug 10;248(15):5549-51.
9
Factors determining the specificity of the tRNA aminoacylation reaction. Non-absolute specificity of tRNA-aminoacyl-tRNA synthetase recognition and particular importance of the maximal velocity.决定tRNA氨酰化反应特异性的因素。tRNA-氨酰-tRNA合成酶识别的非绝对特异性以及最大反应速度的特殊重要性。
Biochimie. 1973 May;55(5):547-57. doi: 10.1016/s0300-9084(73)80415-8.
Zotero 插件