Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, PR China.
Colloids Surf B Biointerfaces. 2011 Jun 15;85(1):8-11. doi: 10.1016/j.colsurfb.2010.10.026. Epub 2010 Oct 20.
The development of methods for DNA detection is of importance in disease diagnosis, gene-targeted drug discovery and molecular biology field. In this paper, we synthesize a new cationic water-soluble CP containing fluorene moiety and flexible ethylenic moiety in the backbone (PFV) for label-free DNA detection. The conformational freedom of PFV provides stronger interactions with double-stranded DNA (dsDNA) and optimizes the orientation of transition moments between PFV and ethidium bromide (EB) intercalated in dsDNA. The efficient FRET from PFV (donor) to EB (acceptor) intercalated in dsDNA is observed and the emission of EB is amplified by the good light-harvesting ability of conjugated polymers. The interactions between PFV and DNA can also be probed by measuring the FRET ratio between PFV and EB intercalated in DNA. In comparison to other DNA detection assays based on FRET and conjugated polymers, synthesis of dye-labeled DNA probe is avoided in our method, which significantly reduces the cost and the synthetic complexity. The PFV/dsDNA/EB system provides promising applications on DNA detection with a simply, fast and label-free manner.
DNA 检测方法的发展在疾病诊断、基因靶向药物发现和分子生物学领域都具有重要意义。在本文中，我们合成了一种新型阳离子水溶性 CP，其主链中含有芴基部分和柔性乙烯基部分（PFV），用于无标记 DNA 检测。PFV 的构象自由度提供了与双链 DNA（dsDNA）更强的相互作用，并优化了 PFV 与嵌入 dsDNA 中的溴化乙锭（EB）之间的跃迁矩的取向。从 PFV（供体）到嵌入 dsDNA 中的 EB（受体）的有效 FRET 被观察到，并且共轭聚合物的良好光收集能力放大了 EB 的发射。PFV 与 DNA 之间的相互作用也可以通过测量 PFV 与嵌入 DNA 中的 EB 之间的 FRET 比来探测。与其他基于 FRET 和共轭聚合物的 DNA 检测方法相比，我们的方法避免了染料标记 DNA 探针的合成，这显著降低了成本和合成复杂性。PFV/dsDNA/EB 体系为 DNA 检测提供了一种简单、快速、无标记的应用前景。
