State Key Laboratory of Rare Earth Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
Langmuir. 2010 Mar 16;26(6):4540-5. doi: 10.1021/la904173j.
By taking advantage of the large signal amplification through efficient fluorescence resonance energy transfer (FRET) from the conjugated polymer to the intercalating dye mediated by DNA, a new strategy for nuclease assay has been developed using conjugated polymer and DNA/intercalating dye complex. The discrimination of DNA before or after digestion by nuclease denotes the universal application of the approach, in which either dsDNA or ssDNA substrates could be used for detecting nuclease activity. This method can be extended to most nucleases by simply changing the substrate DNA. The present method is label-free, rapid, and highly sensitive, with a detection limit much better or at least comparable to previous reports. In addition, this assay is easy to implement for visual detection with the assistance of a UV transilluminator. We reason that a similar strategy can be adopted for the detection of other analytes.
通过利用共轭聚合物与 DNA 介导的嵌入染料之间有效的荧光共振能量转移（FRET）来实现大信号放大，我们开发了一种使用共轭聚合物和 DNA/嵌入染料复合物进行核酸酶分析的新策略。通过核酸酶对 DNA 的消化前后的区分，该方法具有普遍的适用性，其中可以使用双链 DNA 或单链 DNA 底物来检测核酸酶活性。通过简单地改变底物 DNA，该方法可以扩展到大多数核酸酶。该方法具有无标记、快速、高灵敏度的特点，检测限优于或至少可与之前的报道相媲美。此外，该测定法易于借助紫外透射仪进行可视化检测。我们认为，类似的策略可以用于检测其他分析物。
