Key Laboratory of Functional Polymer Materials of Ministry of Education, Institute of Polymer Chemistry, Nankai University, 94# Weijin Road, Tianjin 300071, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Dec 15;878(32):3375-81. doi: 10.1016/j.jchromb.2010.10.023. Epub 2010 Oct 30.
The individual flavonoid component, scutellarin, scutellarein, luteolin and apigenin, in Scutellaria barbata plant was isolated based on the macro porous adsorbent with high adsorption selectivity. These adsorbents were synthesized based on the copolymerization of methyl acrylate and divinylbenzene (MA-co-DVB). So the polarity and the adsorption affinity of these adsorbents can be adjusted through changing MA content in the adsorbents. And then the ability of the adsorbent with different MA contents for isolation of these four individual flavonoid was also investigated. Adsorbents M2 and M4, with MA content of 25% and 45%, respectively, demonstrated the best separation ability. Complete separation of the four flavone compounds was achieved in a continuous process based on combination of adsorbents with different polarities (M2 and M4). Gradient elution using adsorbent M4 separated the four flavonoids into three fractions, which were determined to contain scutellarin, scutellarein and a mixture of luteolin and apigenin. The latter was separated completely by adsorbent M2 subsequently. All four compounds were obtained at high resolution and high recovery yield (96.7%, 94.1%, 95.8% and 93.8%, respectively), suggesting the efficiency of sequentially combined columns with different segregation patterns.
以具有高吸附选择性的大孔吸附剂为基础，从黄芩植物中分离出单体类黄酮成分，包括野黄芩苷、黄芩素、木犀草素和芹菜素。这些吸附剂是通过甲基丙烯酸甲酯和二乙烯基苯（MA-co-DVB）共聚合成的。因此，可以通过改变吸附剂中 MA 的含量来调节这些吸附剂的极性和吸附亲和力。然后，研究了不同 MA 含量的吸附剂对这四种单体类黄酮分离的能力。MA 含量分别为 25%和 45%的吸附剂 M2 和 M4 表现出最佳的分离能力。基于不同极性吸附剂（M2 和 M4）的组合，实现了四种黄酮类化合物的完全分离。使用吸附剂 M4 进行梯度洗脱，将四种黄酮类化合物分离成三个馏分，分别鉴定为野黄芩苷、黄芩素和木犀草素和芹菜素的混合物。随后，用吸附剂 M2 将后者完全分离。所有四种化合物均以高分辨率和高回收率（分别为 96.7%、94.1%、95.8%和 93.8%）获得，表明具有不同分离模式的顺序组合柱的效率。
