Department of Environmental Engineering & Science, Chia Nan University of Pharmacy and Science, Tainan 71710, Taiwan.
J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Dec 1;940:126-34. doi: 10.1016/j.jchromb.2013.09.015. Epub 2013 Sep 25.
A hyphenated procedure of heat-reflux and ultrasound-assisted extraction (HUAE), and an accurate high-performance liquid chromatographic (HPLC) method were developed for the determination of apigenin, baicalin and luteolin content in Scutellaria barbara D. Don. The suitable HUAE conditions for the extraction of target compounds from the herb were identified as an ultrasonic frequency of 40kHz, power of 185W, duty cycle of 75% (intermittent sonication), mean particle size of 0.355mm, extraction temperature of 50°C, ratio of solvent to raw material of 12:1 (mL/g), ethanol concentration of 60% (v/v), extraction time of 30min and three cycles. Compared with a traditional heat-reflux extraction method, the proposed method reduced the extraction time, extraction temperature and solvent consumption. Also, this HUAE method achieved superior apigenin, baicalin and luteolin yields. Furthermore, the developed HUAE-HPLC method was applied successfully for the simultaneous evaluation of three bioactive compounds in five samples of S. barbara D. Don obtained from different geographical regions. These results clearly demonstrated that the combined HUAE-HPLC process is feasible in the future commercialized manufacture of this highly valuable Chinese herbal medicine.
建立了一种回流和超声辅助提取（HUAE）的联合程序，以及一种准确的高效液相色谱（HPLC）方法，用于测定黄芩中的芹菜素、黄芩苷和木犀草素含量。巴戟天。从草药中提取目标化合物的合适 HUAE 条件被确定为超声频率为 40kHz、功率为 185W、占空比为 75%（间歇超声）、平均粒径为 0.355mm、提取温度为 50°C、溶剂与原料的比例为 12:1（mL/g）、乙醇浓度为 60%（v/v）、提取时间为 30min 和 3 个循环。与传统的回流提取方法相比，该方法缩短了提取时间、提取温度和溶剂消耗。此外，该 HUAE 方法实现了更高的芹菜素、黄芩苷和木犀草素产率。此外，所开发的 HUAE-HPLC 方法成功地用于同时评估来自不同地理区域的 5 个黄芩样品中的三种生物活性化合物。这些结果清楚地表明，联合 HUAE-HPLC 工艺在未来商业化生产这种极具价值的中药方面是可行的。
