Department of Molecular Medicine and Pathology, School of Medical Sciences, The University of Auckland, Private Bag 92019, Auckland Mail Centre, Auckland 1142, New Zealand.
Dev Comp Immunol. 2011 Mar;35(3):385-91. doi: 10.1016/j.dci.2010.11.008. Epub 2010 Nov 25.
The zebrafish is increasingly being utilized to study aspects of the conserved innate intestinal immunity of vertebrates. In mammals, some antimicrobial proteins are synthesised by specialised immune cells that appear to have no equivalent in zebrafish. To delineate foci of antimicrobial protein production along the zebrafish intestine, we examined the antero-posterior expression gradients of antimicrobial genes. Quantitative PCR revealed distinct expression gradient profiles, with the mid-intestine exhibiting elevated expression of several genes such as dual oxidase and the defensin beta-like and peptidoglycan recognition protein families. This region also presented with the most numbers of leukocytes and endocytic cells, supporting a specialised immunological role. Conversely, expression of the Dr-RNase family was prominent in the anterior intestine. Expression of the zebrafish β-defensin family was examined in adult zebrafish tissues. Strong expression of defensin beta-like 1 was detected in the swim bladder of zebrafish from the larval stage of development through to adults.
斑马鱼正越来越多地被用于研究脊椎动物保守的固有肠道免疫的各个方面。在哺乳动物中，一些抗菌蛋白是由专门的免疫细胞合成的，而这些细胞在斑马鱼中似乎没有对应物。为了描绘斑马鱼肠道中抗菌蛋白产生的焦点，我们检查了抗菌基因的前后表达梯度。定量 PCR 显示了不同的表达梯度图谱，其中中肠表现出几种基因的表达升高，如双氧化酶和防御素β样和肽聚糖识别蛋白家族。该区域还具有最多数量的白细胞和内吞细胞，支持其具有专门的免疫作用。相反，Dr-RNase 家族的表达在前肠中较为显著。在成年斑马鱼组织中检查了斑马鱼 β-防御素家族的表达。在从幼体发育到成年的斑马鱼的鳔中检测到防御素β样 1 的强烈表达。
