Jia Panpan, Deng Shun, Lin Xiaoxing, Song Liyan, Wang Yangqing, Pei De-Sheng
School of Public Health, Chongqing Medical University, Chongqing 400016, China.
Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China.
Environ Health (Wash). 2023 Sep 7;1(4):258-269. doi: 10.1021/envhealth.3c00072. eCollection 2023 Oct 20.
The prevalence of residual antibiotics is a global threat to human health. Less is known about the potential health effects of residual antibiotics in freshwater systems. Here, zebrafish were used to explore chronic effects of environmentally relevant concentrations (ERCs) of tetracycline (TC). Although chronic exposure to TC did not significantly alter the body weight of adult zebrafish, the exposed zebrafish parents exhibited substantial changes in gut microbiota composition and a reduced gut-weight ratio. Notably, male fish exposed to TC showed a significant decline of critical intestinal function-related metabolites (i.e., triglycerides, glucose, and free fat acid), while this was not observed in females, resulting in sex-dependency. The gut microbial composition of chronically exposed zebrafish parents changed substantially, but the disruption was not transferred to their respective offspring without exposure. However, the perturbation of insulin-related signaling pathways caused by TC exposure was not attenuated in the zebrafish offspring after removal of TC exposure. Taken together, our findings suggest that chronic exposure to TC disturbs gut microbial communities and metabolism and exerts chronic effects on the insulin/IGF-1 mediated signaling cascades, implying that exposure to antibiotics not only leads to the selection of resistant microbes but also poses long-term deleterious health risks for the next generation.
残留抗生素的普遍存在是对人类健康的全球威胁。人们对淡水系统中残留抗生素的潜在健康影响了解较少。在此，使用斑马鱼来探究环境相关浓度（ERCs）的四环素（TC）的慢性影响。尽管长期暴露于TC并未显著改变成年斑马鱼的体重，但暴露的斑马鱼亲代的肠道微生物群组成出现了显著变化，且肠道重量比降低。值得注意的是，暴露于TC的雄鱼显示出关键肠道功能相关代谢物（即甘油三酯、葡萄糖和游离脂肪酸）显著下降，而雌鱼未观察到这种情况，这导致了性别依赖性。长期暴露的斑马鱼亲代的肠道微生物组成发生了显著变化，但这种破坏在未暴露的各自后代中并未传递。然而，在去除TC暴露后，斑马鱼后代中由TC暴露引起的胰岛素相关信号通路的扰动并未减弱。综上所述，我们的研究结果表明，长期暴露于TC会扰乱肠道微生物群落和代谢，并对胰岛素/IGF-1介导的信号级联产生慢性影响，这意味着接触抗生素不仅会导致耐药微生物的选择，还会对下一代造成长期有害的健康风险。
