Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida, Tokyo 194-8511, Japan.
Neurosci Lett. 2011 Jan 20;488(2):199-203. doi: 10.1016/j.neulet.2010.11.029. Epub 2010 Nov 18.
Gangliosides are sialic acid-containing sphingolipids that have promoting effects on the survival, growth, and functions of neurons in the central nervous system. Glial cells including astrocytes assist neurons with regard to their proliferation, maintenance, and function. In the present study, we found that astrocytes with ganglioside depletion caused by treatment with d-threo-1-phenyl-1-2-decanoylamino-3-morpholino-1-propanol (d-PDMP) exhibited reduced neuronal survival activity, as evaluated using low-density hippocampal cultures. The reduction was rescued by a ganglioside, GM3, suggesting that GM3 has significant effects on the glial cell activities that help neurons to proliferate and be maintained.
神经节苷脂是含有唾液酸的鞘脂类，对中枢神经系统神经元的存活、生长和功能具有促进作用。神经胶质细胞包括星形胶质细胞在神经元的增殖、维持和功能方面为其提供帮助。在本研究中，我们发现用 d-threo-1-苯基-1-2-癸酰氨基-3-吗啉基-1-丙醇（d-PDMP）处理耗尽神经节苷脂的星形胶质细胞，会降低使用低密度海马培养物评估的神经元存活活性。通过神经节苷脂 GM3 进行的挽救实验表明，GM3 对有助于神经元增殖和维持的胶质细胞活性具有重要作用。
