Department of Anatomy, Hyogo College of Medicine, Mukogawa, Nishinomiya, Hyogo 663-8501, Japan.
J Chem Neuroanat. 2011 Jan;41(1):55-62. doi: 10.1016/j.jchemneu.2010.11.003. Epub 2010 Nov 17.
We have studied the connections of calcitonin gene-related peptide immunoreactive (CGRP-ir) sensory neurons in the ganglia of the vagus nerve. Many CGRP-ir neurons were identified in the jugular ganglion located in the cranial cavity, while fewer CGRP-ir neurons were found in the nodose ganglion located at the level of the jugular foramen. Application of Fluorogold to the cut end of the cervical vagus nerve resulted in many Fluorogold-labeled neurons in both the jugular and the nodose ganglia. Application of Fluorogold to the cut end of the subdiaphragmatic vagus nerve resulted in Fluorogold-labeled neurons mostly in the nodose ganglion with only a few labeled neurons in the jugular ganglion. Injection of Fluorogold into the heart resulted in Fluorogold-labeled neurons in both the jugular and the nodose ganglia. Double labeling combining CGRP immunohistochemistry and Fluorogold retrograde tracing showed that in cases of both the application of Fluorogold to the cut end of the cervical vagus nerve and the injection of Fluorogold into the heart, about 40% of the Fluorogold-labeled neurons in the jugular ganglion expressed CGRP-like immunoreactivity. These results indicate that many CGRP-ir neurons in the jugular ganglion innervate the cervical and thoracic visceral organs, including the heart, but only a few CGRP-ir neurons project to the abdominal visceral organs.
我们研究了降钙素基因相关肽免疫反应（CGRP-ir）感觉神经元在迷走神经神经节中的连接。在位于颅腔的颈静脉神经节中发现了许多 CGRP-ir 神经元，而在位于颈静脉孔水平的结状神经节中发现了较少的 CGRP-ir 神经元。将 Fluorogold 应用于颈迷走神经的切断端，导致颈静脉和结状神经节中都有许多 Fluorogold 标记的神经元。将 Fluorogold 应用于膈下迷走神经的切断端，导致 Fluorogold 标记的神经元主要位于结状神经节，颈静脉神经节中仅有少数标记神经元。将 Fluorogold 注射到心脏中，导致颈静脉和结状神经节中都有 Fluorogold 标记的神经元。将 CGRP 免疫组织化学和 Fluorogold 逆行追踪相结合的双重标记显示，在将 Fluorogold 应用于颈迷走神经的切断端和将 Fluorogold 注射到心脏的情况下，颈静脉神经节中约 40%的 Fluorogold 标记神经元表达 CGRP 样免疫反应性。这些结果表明，颈静脉神经节中的许多 CGRP-ir 神经元支配颈胸内脏器官，包括心脏，但只有少数 CGRP-ir 神经元投射到腹部内脏器官。
