Bioanalytical and Discovery Analytical Sciences, Research and Development, Bristol-Myers Squibb Company, PO Box 4000, Princeton, NJ 08543, USA.
Drug Discov Today. 2011 Jan;16(1-2):58-64. doi: 10.1016/j.drudis.2010.11.003. Epub 2010 Nov 18.
Mass spectrometry (MS) has become a powerful technology in the discovery and development of protein therapeutics in the biopharmaceutical industry. This review article describes recent developments and future trends in the characterization of protein therapeutics using MS. We discuss top-down MS for the characterization of protein modifications, hydrogen/deuterium exchange MS and ion mobility MS methods for higher order protein structure studies. Quantitative analysis of protein therapeutics (in vivo) by MS as an orthogonal approach to immunoassay for pharmacokinetics studies will also be illustrated.
质谱（MS）已成为生物制药行业中发现和开发蛋白质治疗药物的有力技术。本文综述了使用 MS 对蛋白质治疗药物进行表征的最新进展和未来趋势。我们讨论了用于蛋白质修饰表征的自上而下的 MS、用于高阶蛋白质结构研究的氢/氘交换 MS 和离子淌度 MS 方法。还将举例说明通过 MS 进行蛋白质治疗药物（体内）的定量分析作为免疫分析的正交方法在药代动力学研究中的应用。
