Department of Chemistry, University of Reading, Whiteknights, Reading RG6 6DX, U.K.
Waters Corporation, Stamford Avenue, Wilmslow SK9 4AX, U.K.
J Am Soc Mass Spectrom. 2023 Jun 7;34(6):991-994. doi: 10.1021/jasms.3c00068. Epub 2023 Apr 27.
High-throughput (HTP) mass spectrometry (MS) is a rapidly growing field, with many techniques evolving to accommodate ever increasing sample analysis rates. Many of these techniques, such as AEMS and IR-MALDESI MS, require volumes of at least 20-50 μL for analysis. Here, liquid atmospheric pressure-matrix-assisted laser desorption/ionization (LAP-MALDI) MS is presented as an alternative for ultra-high-throughput analysis of proteins requiring only femtomole quantities of protein in 0.5 μL droplets. By moving a 384-well microtiter sample plate with a high-speed XY-stage actuator, sample acquisition rates of up to 10 samples per second have been achieved at a data acquisition rate of 200 spectra per scan. It is shown that protein mixture solutions with concentrations of ≤2 μM can be analyzed at this speed, while individual protein solutions can be analyzed at concentrations of ≤0.2 μM. Thus, LAP-MALDI MS provides a promising platform for multiplexed HTP protein analysis.
高通量 (HTP) 质谱 (MS) 是一个快速发展的领域，许多技术都在不断发展以适应不断增加的样品分析速率。其中许多技术，如 AEMS 和 IR-MALDESI MS，分析时至少需要 20-50 μL 的体积。在这里，提出了液体常压-基质辅助激光解吸/电离 (LAP-MALDI) MS 作为替代方法，用于需要在 0.5 μL 液滴中仅使用飞摩尔量蛋白质的超高通量蛋白质分析。通过使用高速 XY 台致动器移动 384 孔微孔板，以 200 个光谱/扫描的采集速率，实现了高达每秒 10 个样本的采集率。结果表明，以这种速度可以分析浓度≤2 μM 的蛋白质混合溶液，而对于单种蛋白质溶液，可以分析浓度≤0.2 μM 的溶液。因此，LAP-MALDI MS 为高通量蛋白质分析提供了一个有前景的平台。
